Banca de QUALIFICAÇÃO: RENATA ALVES PESSÔA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : RENATA ALVES PESSÔA
DATA : 25/02/2019
HORA: 09:00
LOCAL: Sala de aula da pós-graduação
TÍTULO:

ASSESSMENT OF THE IMMUNOLOGICAL RESPONSE PROFILE IN ACUTE PHASE PATIENTS IN ZIKA VIRUS INFECTION

PALAVRAS-CHAVES:

Zikavírus, Rt-PCR, adaptive immunity.

PÁGINAS: 53
GRANDE ÁREA: Ciências Biológicas
ÁREA: Microbiologia
RESUMO:

Zika fever virus or ZIKV is an Arbovirus of the genus Flavivirus, family flaviviridae. It has an icosahedral geometric capsid, enveloped and the genetic material is single strand RNA positive polarity. It was first isolated in the year 1947 from an infected Rhesus monkey living in the Zika Forest in Unganda, Africa. Although it was a known arbovirus, it became a public health problem after the first epidemic in 2007 in Microneisaia, later the virus spread to more than 50 countries and happened to be correlated with two serious neurological disorders the congenital syndrome of zika virus, whose main symptom is microcephaly, and GuillianBarré syndrome (GBS). This study aimed to characterize the adaptive immunology response to infection by Zika virus, such as how the body acts in response to the infection, which is the immunological profile, which interleukins and transcription factors are expressed in this micro environment and which defensive cellular patterns are generated in the acute phase of infection considered acute phase the period from 3 to 5 days after the onset of symptoms). The study consisted of a total of 39 samples, 30 peripheral blood samples with confirmatory PCR for ZIKV and 9 samples from healthy individuals. Real-time PCR reactions were performed for both groups to verify the presence of some interleukins and transcription factors in order to characterize the adaptive immune response generated by comparing the analyte concentrations present in the samples from healthy and infected individuals. The results obtained from the PCRs were analyzed and statistically treated in PRISM® 7.0 software. Significant statistical differences were observed for the transcription factors: FoxP3, RoRyT, STAT3, PU1 and GATA3 and for interleukins IL4, IL10 and IL22. No significant changes were observed for: AHAR, IFN, IL9, IL17, INOS and TGF-β. The GATA3 transcription factor was higher for the control group. The combination of the interleukins and transcription factors present in the solution did not lead us to a defined response profile, either by the characteristics of the viral infection in the organism or by the elapsed time of infection (short period for the consolidation of an efficient adaptive immune response). Although we can not point out a response profile: Th1, Th2, Th9, Th17, Th22 or Treg, we can observe that the microenvironment formed is predominantly regulatory.

MEMBROS DA BANCA:
Presidente - 348473 - JOSE VERISSIMO FERNANDES
Externo ao Programa - 1715230 - JOSELIO MARIA GALVAO DE ARAUJO
Externo ao Programa - 1752367 - PAULO MARCOS DA MATTA GUEDES