Banca de DEFESA: JULIETE TAVARES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : JULIETE TAVARES
DATA : 25/05/2018
HORA: 14:00
LOCAL: Auditório do Museu de Ciências Morfológicas - CB
TÍTULO:

EVALUATION OF THE EFFECTS OF SHORT- AND LONG-TERM EFFECTS OF ALCOHOL WITHDRAWAL ON ANXIETY-RELATED BEHAVIORS AND SEROTONINERGIC STAINING IN RAT DORSAL RAPHE NUCLEUS OF RATS

PALAVRAS-CHAVES:

Anxiety. Alcohol. Open field. Dorsal raphe nucleus. Elevated plus maze. Serotonergic system. Withdrawal.

PÁGINAS: 75
GRANDE ÁREA: Ciências Biológicas
ÁREA: Farmacologia
RESUMO:

Abusive use of alcohol is classified as a substance-induced disorder, capable of producing intoxication, withdrawal, and anxiety disorders, possibly leading to relapse. Several studies have demonstrated the role of the serotonergic system as a modulator of behaviors related to anxiety and reward and of many brain regions in the neural substrates of anxiety and alcohol dependence. Among these areas, we can mention Dorsal raphe nucleus and its portions, from which most of the neurons that release 5-HT in various brain areas leave. Therefore, the goal of this study was to investigate whether alcohol would alter the labeling of serotonergic cells in the NDR, specifically in the DRD and DRV portions, in animals submitted to short (3 days) and long term (21 days) withdrawal, as well as continuous consumption and correlate the marking of the cells with behavioral states related to the anxiety generated by alcohol withdrawal. For this, two independent experiments were performed: in experiment 01 (behavioral stage), male Wistar rats were divided into 3 experimental groups, being a control group (water), 3 days withdrawn group and 21 days withdrawn group, all groups received chronic treatment with alcohol for 21 days. After 3 days withdrawal and withdrawal of 21 days, were submitted to the elevated plus maze (EPM). Twenty-four hours later, they were submitted to the open field (OF) where anxiety-related behaviors were assessed for 5 minutes. In the experiment 02 (immunohistochemical analysis) the groups mentioned above were added to the group that received continuous alcohol for 21 days, without withdrawal and followed the same treatment and withdrawal scheme. Then, it was performed a perfusion and then immunohistochemistry test in the DRD and DRV portions of the DRN. The weights and consumption of feed and water-alcohol were monitored throughout the experimental period, for both experiments. In experiment 1, it was observed that alcohol withdrawal for 3 or 21 days did not alter the conventional and ethological parameters related to anxiety in the EPM and OF, nor did it alter the locomotion in EPM. In Experiment 2, it was observed that alcohol withdrawal for 3 or 21 days and continuous consumption did not change in any way meant the immunoblot of serotonergic cells in both the observed portions. However, a significant increase in cell density was observed for the 3 day withdrawn group. There was no change in food intake and water intake during the experimental period, but a significant decrease in body weight was observed when compared to the control. This study demonstrated that serotonergic neurotransmission may be affected by alcohol withdrawal, but this change was not related to anxiety-related behaviors.

MEMBROS DA BANCA:
Presidente - 2412258 - EDILSON DANTAS DA SILVA JUNIOR
Externo ao Programa - 1645202 - ELAINE CRISTINA GAVIOLI
Externo à Instituição - MARIANA FERREIRA PEREIRA DE ARAUJO - IINN