Banca de QUALIFICAÇÃO: CAMILA BANDEIRA GUERRA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : CAMILA BANDEIRA GUERRA
DATA : 13/04/2018
HORA: 14:00
LOCAL: Sala de Reuniões do CB
TÍTULO:

Short and long-term effects of alcohol withdrawn on depression related behavior and serotonin immunoreactivity on rafe median nucleus

PALAVRAS-CHAVES:

Key words: ethanol, abstinence, depression, serotonin, median raphe nucleus, forced swimming test, immunohistochemistry.

PÁGINAS: 77
GRANDE ÁREA: Ciências Biológicas
ÁREA: Farmacologia
SUBÁREA: Neuropsicofarmacologia
RESUMO:

Abstinence from alcohol in dependent individuals promotes a set of physical and psychic signs and symptoms, some of which are related to depression. Depressive behaviors are observed in rats after chronic alcohol consumption followed by withdrawal, which can be observed in the forced swimming test. Several preclinical studies have used animal models of alcohol consumption followed by withdrawal to analyze the changes in neurocircuits resulting from alcohol use. Among the several alterations, there has been a decrease in the serotonin (5-HT) content in the whole brain, especially in the synthesis areas. The purpose of the present study was to determine whether short-term and long-term withdrawals after chronic ethanol consumption would promote behavioral changes suggestive of depression in the open field (CA) test and forced swimming test (TNF), and whether changes in immunostaining of serotonin reactive cells in the median raphe nucleus (MRN). Male Wistar rats with approximately 60 days of life were submitted to increasing concentrations of ethanol as the only source of liquid diet or water, both with free access to feed. In the behavioral stage, after 21 days of exclusive alcohol consumption, ethanol was replaced with water (withdrawal) and, after 72 hours or 21 days of withdrawal, the animals were exposed to the CA test. During this period, the locomotor activity of the animal was evaluated through the parameters of distance traveled and mean velocity. On the following day, TNF was used to evaluate the climbing and immobility times, parameters used to verify depressive type behaviors. In the immunohistochemical stage, the brains of animals submitted to chronic ethanol consumption for 21 days, followed or not by 72-hour or 21-day withdrawal, and their controls, were submitted to immunohistochemistry to verify the labeling of 5-HT immunoreactive cells in the MRN. In addition, the percentage of immunoreactive cell area for 5-HT (optical density) was determined. The behavioral data of CA did not show any alteration in the locomotor activity of the animals in all groups. As for TNF, the data showed decreased climbing time in the long-term withdrawal group and increased immobility time in the short-term withdrawal and long-term withdrawal groups compared to the control group. In immunohistochemistry, there were no differences in the counting of 5-HT immunoreactive cells in the MRN between the control groups compared to the groups chronic consumption, short-term withdrawal and long-term withdrawal. The analysis of the optical density verified alteration in the short-term withdrawal group compared to the control. Taken together, the data obtained demonstrate a depressor-induced effect induced by short- and long-term ethanol withdrawals that is not related to changes in the serotonin labeling in the median raphe nucleus.

MEMBROS DA BANCA:
Presidente - 2412258 - EDILSON DANTAS DA SILVA JUNIOR
Externo ao Programa - 1645202 - ELAINE CRISTINA GAVIOLI
Externo ao Programa - 1076490 - FERNANDO VAGNER LOBO LADD