Banca de QUALIFICAÇÃO: RAFINDRADE GANILSON FERREIRA DJALÓ

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : RAFINDRADE GANILSON FERREIRA DJALÓ
DATA : 15/02/2018
HORA: 14:30
LOCAL: Sala de Reuniões do CB
TÍTULO:

OCCURRENCE OF METHICILLIN-RESISTANT Staphylococcus aureus IN HIV-POSITIVE PATIENTS ATTENDED IN A REFERENCE HOSPITAL IN THE CITY OF NATAL-RN.

PALAVRAS-CHAVES:

Colonization - MRSA - HIV-positive patients

PÁGINAS: 50
GRANDE ÁREA: Ciências Biológicas
ÁREA: Microbiologia
RESUMO:

The bacterial species Staphylococcus aureus is considered one of the most important human pathogens. Furthermore, a notable feature of this species is the ability to acquire antibiotics resistance, the methicillin resistance has been unquestionably significant. Recent studies have shown the presence of methicillin-resistant Staphylococcus aureus (MRSA) in certain population groups, such as HIV-positive patients, which there was a increased the risk of infection by this strain. The aim of the study is to determine the occurrence of MRSA colonizing HIV positive patients treated in a reference hospital in the city of Natal-RN and also to relate the presence of MRSA with the clinical condition associated factors of the individuals. To that end, all HIV-positive patients being treated in the hospital selected for the study were invited to participate and requested to sign the TCLE. A cross-sectional and descriptive study was conducted. The biological samples of the participants were collected for nasal swabs. The samples were seeded in Mannitol Salt agar for S. aureus isolation. The suggestive colonies of this bacterial species were undergo to laboratory tests of identification as Gram staining, bacitracin susceptibility test, catalase test and free coagulase test. The identification of the strain MRSA was performed using the disk diffusion test methodology, the cefoxitin disc was used as a marker, as recommended by the CLSI 2017. The same methodology was used to evaluate the susceptibility to other antimicrobials. The detection of mecA and lukF genes by Polymerase Chain Reaction (PCR) was also performed. The information regarding the clinical condition of the participants was obtained by interview consisting of 16 questions. Of the 400 patients who participated in the study, 129 (32.2%) were colonized by S. aureus. Of these, 9 (2.2%) were resistant to oxacillin, nevertheless only 7 presented the mecA gene and 5 the lukFgene. Most isolates oxacillin resistant showed sensitivity to most of the antibiotics tested, with a high resistance rate only to penicillin G (77.7%). No association was found between the presence of MRSA and the clinical conditions of the  participants evaluated. However, the presence of MRSA lineage, acknowledged for the virulence and the easiness for acquiring mechanisms of antimicrobial resistance, colonizing patients that are more vulnerable to infections may represent a risk factor for this population.

MEMBROS DA BANCA:
Externo à Instituição - ANDRÉ LUIZ BARBOSA DE LIMA - PMN
Interno - 348473 - JOSE VERISSIMO FERNANDES
Presidente - 1452833 - MARIA CELESTE NUNES DE MELO