Banca de DEFESA: JÉSSICA TEIXEIRA JALES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : JÉSSICA TEIXEIRA JALES
DATA : 16/02/2018
HORA: 09:00
LOCAL: A definir
TÍTULO:

USE OF MEDIUM INFRARED SPECTROSCOPY (ATR-FTIR) IN IDENTIFICATION OF Trypanosoma cruzi in Triatoma brasiliensis EXPERIMENTALLY INFECTED

PALAVRAS-CHAVES:

Trypanosoma cruzi; Triatoma brasiliensis; Biospectroscopy. ATR-FTIR

PÁGINAS: 64
GRANDE ÁREA: Ciências Biológicas
ÁREA: Parasitologia
RESUMO:

Trypanosoma cruzi infection is a public health problem and it is substancial that its diagnosis be performed quickly and efficiently. However, the techniques applied on the diagnosis of T. cruzi infection present low sensitivity and specificity regarding the entomological and epidemiological surveillance of the parasite. Considering this problem, the biospectroscopy with the use of ATR-FTIR was used in this study to evaluate its efficiency in the identification of the experimental infection of Triatoma brasiliensis by different DTUs of T. cruzi. The standardization of the calibration model included the use of fourth and fifth instars of T. brasiliensis, which were infected with different DTUs of the parasite (TcI, TcII, TcIII or mixed infection) at a concentration of 40,000 parasites / ml of blood. Followed 15 and 30 days after infection (DAI), infrared spectra were collected from the abdomen of each insect; 30 DAI, intestinal wastes were collected and later used for kDNA analysis. The standardization of the calibration model showed that the application of the mild-infrared using the ATR-FTIR distinguished the triatomines that were not infected and experimentally infected with T. cruzi. In addition, the technique was able to identify the DTUs used, separating them into 5 distinct groups only with the exploratory data analysis through the application of Principal Component Analysis (PCA), which differentially identified protein clusters, nucleic acids and to a lesser extent, lipids. Thus, biospectroscopy applied with the use of ATR-FTIR was efficient in the identification of the T. brasiliensis infection by T. cruzi, and could be used as a diagnostic tool for the future, assisting in the entomological surveillance of the infection by this parasite.

MEMBROS DA BANCA:
Externo ao Programa - 1879211 - BRUNO CAVALCANTE BELLINI
Externo ao Programa - 1714946 - KASSIO MICHELL GOMES DE LIMA
Externo à Instituição - PATRICIA BATISTA BARRA MEDEIROS BARBOSA - UERN
Presidente - 1715271 - RENATA ANTONACI GAMA