Banca de QUALIFICAÇÃO: JÉSSICA TEIXEIRA JALES

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : JÉSSICA TEIXEIRA JALES
DATA : 28/12/2017
HORA: 09:00
LOCAL: Sala de Reuniões do CB
TÍTULO:

USE OF MEDIUM INFRARED SPECTROSCOPY (ATR-FTIR) FOR
IDENTIFICATION OF Trypanosoma cruzi in Triatoma brasiliensis
EXPERIMENTALLY INFECTED

PALAVRAS-CHAVES:

Trypanosoma cruzi; Triatoma brasiliensis; Biospectroscopy.
ATR-FTIR.

PÁGINAS: 64
GRANDE ÁREA: Ciências Biológicas
ÁREA: Parasitologia
RESUMO:

Trypanosoma cruzi infection has a chronic nature, high morbidity and a wide
geographic distribution, so it is substancial that its diagnosis be performed quickly and
efficiently. However, the techniques applied on the diagnosis of T. cruzi infection present
low sensitivity and specificity regarding the entomological and epidemiological
surveillance of the parasite. Considering this problem, the biospectroscopy with the use of
ATR-FTIR was used in this study to evaluate its efficiency in the identification of the
experimental infection of Triatoma brasiliensis by different DTUs of T. cruzi. The
standardization of the calibration model included the use of fourth and fifth instars of T.
brasiliensis, which were infected with different DTUs of the parasite (TcI, TcII, TcIII or
mixed infection) at a concentration of 40,000 parasites / ml of blood. Followed 15 and 30
days after infection (DAI), infrared spectra were collected from the abdomen of each insect;
30 DAI, intestinal wastes were collected and later used for kDNA analysis. The
standardization of the calibration model showed that the application of the mild-infrared
using the ATR-FTIR was efficient, simple and accurate in the identification of triatomines
not infected and experimentally infected with T. cruzi. In addition, the technique was able
to distinguish the DTUs used, separating them into 5 distinct groups only with the
exploratory data analysis through the application of Principal Component Analysis (PCA),
which differentially identified protein clusters, nucleic acids and to a lesser extent, lipids.
Thus, biospectroscopy applied with the use of ATR-FTIR was efficient in the identification
of the T. brasiliensis infection by T. cruzi, and could be used as a diagnostic tool for the
future, assisting in the entomological surveillance of the infection by this parasite.

MEMBROS DA BANCA:
Externo ao Programa - 1218940 - ANTONIA CLAUDIA JACOME DA CAMARA
Externo ao Programa - 1752367 - PAULO MARCOS DA MATTA GUEDES
Presidente - 1715271 - RENATA ANTONACI GAMA