Banca de DEFESA: BRUNA COSTA MOURA DO VALE

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : BRUNA COSTA MOURA DO VALE
DATA : 29/06/2017
HORA: 09:00
LOCAL: Auditório do Museu de Ciências Morfológicas - CB - UFRN
TÍTULO:

DECREASED SUSCEPTIBILITY TO CHLORHEXIDINE AND DISTRIBUTION OF QACA/B GENES AMONG COAGULASE-NEGATIVE STAPHYLOCOCCUS CLINICAL SAMPLES.

PALAVRAS-CHAVES:

Coagulase-negative Staphylococci, Chlorhexidine, Health care-associated infections,

qacA/B genes.

PÁGINAS: 94
GRANDE ÁREA: Ciências Biológicas
ÁREA: Microbiologia
RESUMO:

Health Care Associated Infections (HAI) are currently a of major public health

problem. The coagulase-negative Staphylococci (CNS) are among the most prevalent

species in this type of infection due to their ability to colonize invasive medical devices.

Chlorhexidine is extensively used in the hospital setting as a means of prevention and

control of HAI. However, the existence of strains with reduced susceptibility caused

possibly by the presence of the qacA/B genes has been reported in the literature. The main

of this study was to investigate the susceptibility to chlorhexidine and its relation to the

presence of the qacA/B genes in 211 clinical samples of ECN from diferente hospitals in

the city of Natal-NR during the period from 2010 to 2015. Samples were identified by

standard biochemical tests; the susceptibility to antimicrobials was performed using the

disc-diffusion methodology. The susceptibility to chlorhexidine was evaluated by

determining the minimum inhibitory concentration (MIC) by the agar dilution technique.

The PCR method was used to investigate the qacA/B genes in 32 resistant samples and 32

susceptible samples to chlorhexidine and mecA gene. In total of 211 CNS samples, 69%

were isolated from blood cultures. The most frequent species were S. epidermidis, S.

hominis hominis, S. auricularis and S. haemolyticus, respectively. The strains had a

multidrug resistance profile of 87%, including resistance to oxacillin; 31% demonstrated

reduced susceptibility to chlorhexidine. The qacA/B genes were detected in both

chlorhexidine resistant (32/32) and susceptible samples (17/32). Most of the strains with

reduced susceptibility to chlorhexidine also showed multidrug resistance to the tested

antibiotics (94%). Identification of strains with reduced susceptibility to chlorhexidine in

this study poses a threat to HAIs control practices. Although all strains resistant to

chlorhexidine had the qacA/B genes, their presence was not a determining factor for the

expression of the resistance, requiring additional studies. The decrease in susceptibility to

antiseptics may allow bacteria to persist in the hospital environment, thus contributing to

the survival of multiresistant strains.

5. Palavras-chave (português):

Estafilococos coagulase-negativa, Clorexidina, Infecções

MEMBROS DA BANCA:
Externo à Instituição - ELIZABETH CRISTINA GOMES DOS SANTOS - FANEC
Presidente - 1452833 - MARIA CELESTE NUNES DE MELO
Interno - 1714290 - RENATO MOTTA NETO