Banca de DEFESA: LEONARDO CESAR ANDRIOLA CARVALHO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : LEONARDO CESAR ANDRIOLA CARVALHO
DATA : 19/05/2017
HORA: 15:00
LOCAL: Auditório do Museu de Ciências Morfológicas
TÍTULO:

Etiology and resistance of isolated bactéria of external otitis in dogs

PALAVRAS-CHAVES:

Canine external otitis, antimicrobial resistance, tests of bacterial resistance

PÁGINAS: 84
GRANDE ÁREA: Ciências Biológicas
ÁREA: Microbiologia
RESUMO:

The canine external otitis is a relevant disease regarding veterinary clinic. Considering the

high frequency of treatment of dogs with external bacteria otitis, this research aims to

identify the etiological frequency of otopathy and trace the resistance profile of the

Methicillin Resistant Staphylococcus (MRS) and enterobacteria that produce Extended

Spectrum Beta-Lactamase (ESBL) in outpatients and internment patients. 140 samples of

canine otic exudate were submitted to primary isolation, tests of antimicrobial susceptibility

and bacterial resistance. The isolated groups were: Staphylococcus sp. (122 – 49.38%),

Pseudomonas sp. (22 – 8.91%), enterobacteria (19 – 7.69%), Enterococcus sp. (17 –

6.88%) and Acinetobacter sp. (12 – 4.86%). For the Staphylococcus sp. the Disk Test was

applied along with the resistance test to Methicillin (Met-25). In the positive strains for

Met-25, a Polimerase Chain Reaction (PCR) was applied to detect the mecA gene. For the

PCR positive strains, a Minimum Bactericidal Concentration (MBC) of chlorhexidine

gluconate was determined. 14 MRS strains (11.47%) were observed and all off them had

the mecA gene. 14 Staphylococcus sp. were Disk Test positive. Concerning the positive

Staphylococcus coagulase, the MBC was 500.00µg/mL (0.5%) whereas for the negative

Staphylococcus coagulase the MBC value was 62.500µg/mL (0.0625%). 3 ESBL

enterobacteria strains were detected. The more effective antimicrobial for the

Staphylococcus sp. were: Linezolid (100%), Rifampicin (94.26%) and Chloramphenicol

(92.62%). Regarding the Pseudomonas sp.: Cefepime (100%), Imipenem (100%) and

Meropenem (100%). Regarding the enterobacteria: Ertapenem (100%), Imipenem (100%)

and Cefotaxime (84.21%). Regarding the Enterococcus sp.: Chloramphenicol (100%),

Linezolid (94.12%) and Vancomycin (88.24%). Regarding the Acinetobacter sp.:

Imipenem (100%), Meropenem (100%) and Ticarcillin with Clavulanate (91.67%).

Although the mecA gene and the enterobacteria that produce ESBL are briefly presented in

this research, there is a need for constant monitoring of isolated strains in domestic animals.

The bacteriological growth and the susceptibility tests in the antimicrobial agents are of

great importance in the veterinary clinic routine in order to insure the success in treating

canine otological infections.

MEMBROS DA BANCA:
Interno - 1715271 - RENATA ANTONACI GAMA
Presidente - 1714290 - RENATO MOTTA NETO
Externo à Instituição - WAGNNER JOSÉ NASCIMENTO PORTO - UFAL