Banca de QUALIFICAÇÃO: LEONARDO CESAR ANDRIOLA CARVALHO

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : LEONARDO CESAR ANDRIOLA CARVALHO
DATA : 28/03/2017
HORA: 17:00
LOCAL: Sala de Reuniões do CB
TÍTULO:

ETIOLOGY AND RESISTANCE OF ISOLATED BACTERIA OF EXTERNAL OTITIS IN DOGS

PALAVRAS-CHAVES:

Canine external otitis, antimicrobial resistance, tests of bacterial resistance

PÁGINAS: 92
GRANDE ÁREA: Ciências Biológicas
ÁREA: Microbiologia
RESUMO:

The canine external otitis is a relevant disease regarding veterinary clinic. Considering the high frequency of treatment of dogs with external bacteria otitis, this research aims to identify the etiological frequency of otopathy and trace the resistance profile of the Methicillin Resistant Staphylococcus (MRS) and enterobacteria that produce Extended Spectrum Beta-Lactamase (ESBL) in outpatients and internment patients, 140 samples of canine otic exudate were submitted to primary isolation, tests of antimicrobial susceptibility and bacterial resistance. The isolated groups were: Staphylococcus sp. (122 – 49,38%), Pseudomonas sp. (22 – 8,91%), enterobacteria (19 – 7,69%), Enterococcus sp. (17 – 6,88%) and Acinetobacter sp. (12 – 4,86%). For the Staphylococcus sp. the Disk Test was applied along with the resistance test to Methicillin (Met-25). In the positive strains for Met-25, a Polimerase Chain Reaction (PCR) was applied to detect the mecA gene. For the PCR positive strains, a Minimum Bactericidal Concentration (MBC) of chlorhexidine gluconate was determined. 14 MRS strains (11,47%) were observed and all off them had the mecA gene. 14 Staphylococcus sp. were Disk Test positive. Concerning the positive Staphylococcus coagulase, the MBC was 500.00µg/mL (0,5%) whereas for the negative Staphylococcus coagulase the MBC value was 62.500µg/mL (0,0625%). 3 ESBL enterobacteria strains were detected. The more effective antimicrobial for the Staphylococcus sp. were: Linezolid (100%), Rifampicin (94,26%), Chloramphenicol (92,62%), Cefoxitin (88,53%), Gentamycin (81,15%), Levofloxacin (79,51%), Erytromycin (74,59%) and Clyndamicin (72,95%). Regarding the Pseudomonas sp.: Cefepime (100%), Imipenem (100%), Meropenem (100%), Tobramycin (100%), Levofloxacin (100%), Ceftazidime (95,46%), Aztreonam (95,46%) and Amoxicilin with Clavulanate (95,46%). Regarding the enterobacteria: Ertapenem (100%), Imipenem (100%), Cefotaxime (84,21%), Ceftazidime (84,21%), Cefepime (84,21%), Aztreonam (84,21%), Amoxiciline with Clavulanate (84,21%), Levofloxacin (84,21%), Chloramphenicol (84,21%), Sulfamethoxazole with Trimethroprim (78,95%), Cefoxitin (73,68%), Tobramycin (73,68%) and Amikacin (73,68%). Regarding the Enterococcus sp.: Chloramphenicol (100%), Linezolid (94,12%), Vancomycin (88,24%) Lovofloxacin (88,24%), Peniciline (76,47%), Erytromycin (76,47%) and Ampicillin (76,47%). Regarding the Acinetobacter sp.: Imipenem (100%), Meropenem (100%), Piperacillin with Tazobactam (91,67%), Ticarcillin with Clavulanate (91,67%), Ceftazidime (91,67%), Ceftriaxone (91,67%), Cefepime (91,67%), Tobramycin (91,67%), Amikacin (91,67%), Levofloxine (83,33%) and Sulfamethoxazole with Trimethroprim (75%). Although the mecA gene and the enterobacteria that produce ESBL are briefly presented in this research, there is a need for constant monitoring of isolated strains in domestic animals. The bacteriological growth and the susceptibility tests in the antimicrobial agents are of great importance in the veterinary clinic routine in order to insure the success in treating canine otological infections.

MEMBROS DA BANCA:
Presidente - 1714290 - RENATO MOTTA NETO
Interno - 1715271 - RENATA ANTONACI GAMA
Externo ao Programa - 1714262 - LILIAN GIOTTO ZAROS DE MEDEIROS