Banca de DEFESA: FERNANDA CRISTINA SILVA DE OLIVEIRA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : FERNANDA CRISTINA SILVA DE OLIVEIRA
DATE: 28/08/2025
TIME: 16:00
LOCAL: DEPARTAMENTO DE EDUCAÇÃO FISICA
TITLE:

NFLUENCE OF REPEATED SPRINT EXERCISE ON LEUKOCYTE MORPHOLOGY IN ADOLESCENT ATHLETES WITH DIFFERENT RATES OF BIOLOGICAL MATURATION

KEY WORDS:

Immunology, Lymphocytes, Children, Sports, Biological Maturation Rates.

PAGES: 47
BIG AREA: Ciências da Saúde
AREA: Educação Física
SUMMARY:

Exercise immunology seeks to clarify how different training sessions shape the immune system of athletes in different age groups. In intermittent sports, such as basketball or soccer, athletes routinely include repeated sprint exercises (ESR) in their training routine. ESR, because it is exhausting, can trigger systemic inflammation and, in turn, alter the body's immune responses. It is important to emphasize that, even among pediatric athletes of the same chronological age, the stages of biological maturation can be quite different. Thus, this Master's dissertation aimed to verify the influence of ESR exercise on the leukocyte morphology of pediatric athletes at different biological maturation (BM) rates assessed by skeletal age. Thus, the dissertation was composed of two scientific articles, a literature scoping review and an experimental trial. In the scoping review, we grouped scientific evidence about the immunology of physical exercise in children and adolescent athletes, considering its interaction with biological maturation. Subsequently, for the experimental study, 21 male pediatric athletes performed an ESR session (supramaximal sprints 3x6 with 5 minutes of rest between blocks and 10 seconds of rest between sprints). In pediatrics, BM was analyzed by skeletal age. Peripheral blood samples were collected before, immediately, and 2 hours after ESR. We analyzed leukocyte morphology by optical microscopy and leukogram by automated cell counting. The findings of the literature review indicated that acute exercise causes transient changes in the immune system, such as an increase in leukocyte, lymphocyte, and NK cell counts immediately after exercise, followed by normalization in the recovery phase. Biological maturation proved to be a determining factor, since immunological responses such as increased neutrophil levels, reduced lymphocyte levels or changes in NK cells were more pronounced in post-pubertal individuals than in pre-pubertal individuals. Regarding grouping based on biological maturation, few studies used puberty markers such as Tanner stages; most divided the sample into pre-pubertal and post-pubertal based on chronological age. The findings of the experimental trial indicated that individuals in synchronized BM rhythm exhibited higher levels of total leukocytes and segmented neutrophils immediately after and 2 hours after ESR, compared to their peers in accelerated MB rhythm (p < 0.05), up to 2h after ESR for the group in synchronized MB rhythm systemic inflammation measured through NLR was 50% higher compared to the moment before ESR (p < 0.05), in the group in accelerated MB rhythm 2h after ESR there was a 20% increase for NLR compared to the pre moment (p < 0.05), the sub-analyses the type of sport did not demonstrate a significant effect on total leukocyte levels (η²p: < 0.10, p > 0.05). There was no significant difference between the types of sport practiced in relation to the other variables analyzed (η²p: < 0.13, p > 0.05). This allows us to infer that the influence of repeated sprint exercise on leukocyte morphology in pediatric athletes is dependent on the pace of biological maturation.

Keywords: Immunology, Lymphocytes, Children, Sports, Biological Maturation Rates.

COMMITTEE MEMBERS:
Presidente - 2626634 - PAULO MOREIRA SILVA DANTAS
Interno - 1793257 - ARNALDO LUIS MORTATTI
Interno - 2627140 - BRENO GUILHERME DE ARAUJO TINOCO CABRAL
Externo à Instituição - FELIPE JOSÉ AIDAR MARTINS - UFS
Externo à Instituição - FRANCISCO EMILIO SIMPLICIO DE SOUZA - UERN