Banca de DEFESA: JÚLIA GABRIELA RAMOS PASSOS

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : JÚLIA GABRIELA RAMOS PASSOS
DATE: 27/05/2024
TIME: 14:00
LOCAL: Videoconferência
TITLE:

Estudo de estabilidade térmica e efeito antiofídico de um gel contendo Jatropha mollissima (Pohl) Baill. frente à toxicidade local da peçonha de Bothrops erythromelas

KEY WORDS:

Jatropha mollissima; Bothrops erythromelas; Topical formulation; Stability; Release profile;

PAGES: 129
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Data from the Ministry of Health indicate that Bothrops erythromelas is the
primary cause of snakebite envenomation in Northeast Brazil. Although
antivenom serum is the standard treatment, its limitations include ineffectiveness
in addressing local effects, accessibility issues, and high costs. Plants of the
genus Jatropha (Euphorbiaceae) are utilized in folk medicine to treat the effects
of snakebites. Accordingly, the objective of this study is to incorporate the
hydroethanolic extract of J. mollissima leaves into a semi-solid formulation for
topical use, investigate its thermal stability for a period of 180 days, and evaluate
the antivenom potential of this formulation. The study aims to develop new, more
accessible, and rapid alternatives for treating bites from venomous animals.
The methodology of this study was divided into four parts:
I. Characterization of the extract using High-Performance Liquid Chromatography
(HPLC);
II. Incorporation of the extract into a semi-solid formulation, characterization of
the chemical profile of this formulation by HPLC, and evaluation of its release
profile using the dialysis membrane method;
III. Physicochemical characterization and thermal stability of the formulation for
180 days, assessing organoleptic characteristics, viscosity, pH, weight loss, and
phenolic content using the Folin-Ciocalteu method over time under two
temperature conditions (25 ± 2°C and 40 ± 2°C);
IV. Evaluation of the antivenom activity of the formulation containing the extract
through in vivo inhibition tests (paw edema, cutaneous hemorrhage, and
dermonecrosis) and in vitro inhibition tests using the free extract (phospholipase
A2 activity, proteolytic activity, and hyaluronidase activity).
Results indicated that the formulation exhibited a chemical profile identical to that
of the free extract, revealing the presence of nine major compounds, including
vitexin, cinnamic derivatives, and flavonoids derived from luteolin and apigenin.
The Korsmeyer-Peppas model most accurately represented the extract's release
profile. Stability studies revealed no significant changes in the evaluated
parameters under the two temperature conditions, indicating that the gel remains
stable and safe for up to six months after manufacture. In terms of antivenom
activity, in vivo results demonstrated that the herbal gel effectively inhibited all
tested local effects, likely due to the free extract's effective inhibition of
phospholipase A2, proteases, and hyaluronidases present in the venom, as
demonstrated in in vitro models.
In conclusion, the results indicate that the developed herbal gel is stable and safe
under the evaluated conditions and possesses antivenom potential, effectively
addressing the local effects of venom. This suggests that the topical formulation
has significant antivenom potential as a complementary alternative for treating
local effects induced by bothropic envenoming.

COMMITTEE MEMBERS:
Externa ao Programa - 2374605 - AURIGENA ANTUNES DE ARAUJO - UFRNExterna ao Programa - 1143827 - JULIANA FELIX DA SILVA - nullExterno ao Programa - 2276354 - LEANDRO DE SANTIS FERREIRA - UFRNExterno à Instituição - LUIZ ALBERTO LIRA SOARES - UFPE
Presidente - 1544647 - MATHEUS DE FREITAS FERNANDES PEDROSA
Externa à Instituição - NATHALIA KELLY DE ARAUJO - IFRO