Banca de DEFESA: ANDRE LUIZ SILVA DAVIM

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : ANDRE LUIZ SILVA DAVIM
DATA : 23/02/2017
HORA: 09:00
LOCAL: Auditorio do NUPRAR
TÍTULO:

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PALAVRAS-CHAVES:

bullfrog oil; Microemulsified system, sepsis; inflammation; Toxicity

PÁGINAS: 121
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
RESUMO:

ABSTRACT: Current costs of treating patients with systemically progressing
inflammatory diseases, especially sepsis, are the leading cause of death in non
cardiological intensive care units worldwide. In Brazil, the incidence of sepsis in
patients is becoming more frequent. Despite pharmacological, technological
and surgical advances, mortality due to sepsis and/or associated diseases
remains high worldwide, hence the search for easily accessible and inexpensive
therapeutic alternatives to contain the progression of this disease. Natural
products have played an important role in the pharmaceutical industry because
some of these substances act in a beneficial way on the human immune
system. In recent times, there is a growing investigation of the possible
biological and therapeutic properties attributed to bullfrog oil, since this oil has
been used indiscriminately by the population in the treatment of various
diseases, such as bronchitis, asthma, lichen sclerosus, furunculosis, sebaceous
cyst, and for healing of skin and mucous membranes. However, the possible
consequences of excessive consumption of this oil are increased production of
eicosanoids derived from proinflammatory arachidonic acid and deficiency in
hepatic regulation, predisposing to steatosis, which may progress to hepatic
inflammation and fibrosis. Many hypotheses are based on the performance of
the compounds present in bullfrog oil in the modulation of the inflammatory
response, thus preventing the onset of tissue injuries. The microemulsion is
presented as a possible alternative to a new drug delivery system, in order to
reduce the incidence of hepatotoxicity and protect the organism against the
installation of tissue lesions as a function of the septic condition. The present
study is aimed to evaluate the anti-inflammatory potential of pure bullfrog oil and
a microemulsion system in an experimental model. In this study, a
microemulsified system (WIV) was determined and characterized, and applied
in biological tests to evaluate the anti-inflammatory potential of pure oil and
microemulsion. We used the sepsis model, induced by cecal ligant puncture
(CLP) technique, and the formalin-induced muscle injury. The tests were
performed in murine models, where the animals were randomly separated into
groups and treated with pure bullfrog oil and microemulsion, using the gavage
technique for further evaluation of the hepatotoxic and anti-inflammatory
potential. For the analysis of the anti-inflammatory potential in a sepsis model,
bronchoalveolar lavage was performed with subsequent inflammatory cell
counts and histopathological analyzes of lung tissue. For the analysis in the
muscle injury model, the horizontal extension of the limbs of the animals was
evaluated, as well as the histopathological analysis of the muscle tissue. For the
analysis of the hepatotoxic potential of the substances, the post-sepsis survival
rate and the histopathological analyzes of the hepatic tissues of the animals
were evaluated. When evaluating the toxicity of pure bullfrog oil and in a
microemulsion system, it was observed that in the group that received the microemulsion (ME), the liver had the architecture preserved, but with clinical
signs of hepatic steatosis, unlike the pure bullfrog oil group, which presented
multiple outbreaks of hepatocytic necrosis accompanied by polymorphonuclear
infiltrates. These findings indicate a picture of steatohepatitis, that is, a more
advanced stage and a precursor of hepatic carcinoma. When evaluating the
survival of the animals, it was observed that the ME group survival rate was
significantly higher when compared to the oil group. When evaluating the anti
inflammatory potential of the ME and the oil group in a sepsis model, the
potential for modulation of the inflammatory response was observed in both
groups, since the leukocyte migration to the lungs was significantly reduced
(P<0.01) after the induction of sepsis. When analyzing the histology of the lung
tissues of the animals of both groups, intense wear was observed in the animals
of the oil group, when compared with the ME group, where there was little
tissue compromise. In the muscle injury test, it was observed that the ME and
oil group had good anti-nematode potential until the second hour of injury
induction, when compared to the control group (P<0.01), but no significant
differences were observed between the two groups until twenty-fourth hour
post-injury. In the histological analyzes, greater wear was observed in the oil
group muscle tissue, with an intense presence of cellular infiltrate (edema) and
muscle fibers involvement, and the same intensity of injury was not observed in
the ME group. Thus, it is concluded that pure bullfrog oil and microemulsion
system present good anti-inflammatory potential in the evaluated models,
although the pure oil showed high hepatotoxic potential, characterizing that in a
microemulsified system, it is shown with a possible new drug delivery system
(NSLF).

MEMBROS DA BANCA:
Presidente - 2203888 - MARCIA RODRIGUES PEREIRA
Externo ao Programa - 1149356 - ELIZEU ANTUNES DOS SANTOS
Externo ao Programa - 347057 - TEREZA NEUMA DE CASTRO DANTAS
Externo à Instituição - MARIA APARECIDA MEDEIROS MACIEL - UnP
Externo à Instituição - RICHARD HALTI CABRAL - USP