Banca de DEFESA: BRENA KARISA CAMPOS DE MELO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : BRENA KARISA CAMPOS DE MELO
DATE: 04/02/2022
TIME: 14:30
LOCAL: Sala Remota
TITLE:

EXPERIMENTAL ANTIMALARIAL CHEMOTHERAPY WITH DEHYDRATED COCOS NUCIFERA BEE POLLEN EXTRACTS

KEY WORDS:

Malaria. Bee pollen. Plasmodium berghei ANKA. antimalarial drugs

PAGES: 90
BIG AREA: Ciências Biológicas
AREA: Parasitologia
SUBÁREA: Protozoologia de Parasitos
SPECIALTY: Protozoologia Parasitária Animal
SUMMARY:

Malaria is an acute infectious tropical disease caused by protozoa of the genus Plasmodium, with five species causing malaria in humans, P. falciparum, P. vivax, P. malariae, P. ovale and P. knowlesi, in 2020 there were 241 million cases of malaria in the world, thus remaining one of the main infectious diseases on the planet. Many researches are carried out in search of new drugs for the treatment of the disease, due to the limitations of existing antimalarials, such as parasite resistance. The therapeutic use of medicinal plants has long contributed to the treatment of diseases, and in relation to malaria many drugs such as chloroquine are derived from plants. Therefore, studies in relation to bioactive compounds from plants have shown activity of interest such as anti-inflammatory, antimicrobial, antifungal, anticancer and insecticide. In the present work we seek to investigate the antiplasmodial potential of the extract of bee pollen from Cocos nucifera. For the analysis of parasitemia suppression, female Swiss mice weighing 27 ± 2 g at 6 to 10 weeks of age were used, inoculating 1x106 of red blood cells parasitized by Plasmodium berghei ANKA and separating them into four groups with 5 animals in each, treated orally “per gavage” for 4 consecutive days, negative control group with water, test group with 250 mg/kg, 500 mg/kg and 1000 mg/kg, with bee pollen extract, and positive control group with 25 mg/k of chloroquine. The antimalarial activity was evaluated by the inhibition of the growth of the parasite against the pollen extract in relation to the control group, in addition to the evaluation of cumulative mortality, acute toxicity of the extract in vivo, histopathological analysis, and in vitro analysis, as cytotoxic activity of the extract. , with HepG2 cells being incubated with the extract at six concentrations, from 0.01 to 1000 μg/mL for 24 h. Analyzes of the chemical composition of the extract by GC/MS and LC/MS were performed. The results revealed the presence of fatty acids, coumarins, flavonoids and terpenes. In the acute toxicity assay, we did not observe signs of toxicity and mortality, as well as the histopathological analyzes showed no change in relation to the control. In in vitro toxicity, it was possible to observe that at all concentrations the extract managed to maintain 100% cell viability. The antiplasmodial assays demonstrated the suppression of parasitaemia at concentrations of 500 mg/kg and 1000 m/kg, with values of 49% and 57%, respectively. Despite the favorable results, complementary studies are needed to isolate the active principles to know if there is an interaction between compounds influencing their biological activity.

BANKING MEMBERS:
Presidente - 2213126 - VALTER FERREIRA DE ANDRADE NETO
Externo ao Programa - 1715109 - DANIEL DE LIMA PONTES
Externa à Instituição - CLARICE ABRAMO - UFJF