Banca de QUALIFICAÇÃO: JOHN WESLLEY LIRA SANTOS

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : JOHN WESLLEY LIRA SANTOS
DATE: 29/03/2024
TIME: 09:30
LOCAL: videoconferencia - https://meet.google.com/wev-soar-yhe
TITLE:

Effect of simulated microgravity using clinostat 2D in Lemna aequinoctialis Welw.

KEY WORDS:

Aqueous extract, antioxidant, phenolics, bradford, reducing power, biochemical activity.

PAGES: 54
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUBÁREA: Biologia Molecular
SUMMARY:

The effect of microgravity on plants is not well understood, often varying according to the plant species being studied. In this study, we aimed to evaluate the effect of simulated microgravity on Lemna aequinoctialis plants. This species belongs to the subfamily of water lentils (Lemnoideae) and it has been considered important for bioremediation and as food for fish, pigs, and aquatic birds. Thus, here L. aequinoctialis plants were exposed to simulated microgravity using a 2D clinostat. These assays involved three different rotations per minute (15, 30, 45 rpm) besides a control condition (0 rpm) and these materials were submitted to two different time treatment: 2 hours or 4 hours. After the assay, plant was analyzed during different time as 0, 5, and 10 days. It was measured the protein amount, some antioxidant parameter such as total antioxidant capacity (TAC) and reducing power, as well as the phenolic compounds quantification. For biochemical analysis, aqueous extracts were prepared, and the effects of microgravity were measured. Antioxidant analyses revealed an increase of up to 3 times greater for TAC and reducing power on the 4-hour treatment. Total phenolic compound assay showed an increase value for 45 rpm rotations at 2 hours on days 5 and 10, and at 30 rpm treatment for 4 hours on day 0. Protein quantification showed an increased with values ranging up to  7 times higher to  control at 45 rpm for two hours treatment. Based on the results, it would be important to evaluate the alpha-tocopherol measurement and the level of auxin hormone as well as to measure two antioxidant enzymes as catalase and guaiacol peroxidase. These data are important to understand how Lemna aequinoctialis plants respond to microgravity and use this as a potential tool to improve the quality and increase the biotechnological applications for agriculture, biotechnology, and space exploration.

COMMITTEE MEMBERS:
Presidente - 1720860 - VANESSA DE PAULA SOARES RACHETTI
Externa ao Programa - ***.423.414-** - GÉSSICA LAIZE BERTO GOMES - INSA
Externa ao Programa - ***.574.694-** - NATHALIA MAÍRA CABRAL DE MEDEIROS - NÃO INFORMADO