Banca de QUALIFICAÇÃO: DYONATAN FONSECA SILVA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : DYONATAN FONSECA SILVA
DATE: 09/01/2024
TIME: 14:00
LOCAL: Videoconferencia - Link para acesso: https://meet.google.com/ine-oynw-vqg
TITLE:

EVALUATION OF THE ZYMOGRAPHIC PROFILE OF METALLOPROTEINASES IN A MURINE MODEL OF CHAGAS DISEASE

KEY WORDS:

MMP-9, MMP-2, Electrophoresis, Chronic Chagas heart disease, Polynomial equation

PAGES: 83
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUMMARY:

The etiological agent of Chagas disease is the parasite Trypanosoma cruzi (T. cruzi), characterized by two clinical phases: acute and chronic. Throughout the disease, various cells undergo altered gene expression to favor parasite infection. Consequently, dysregulated expression of cytokines and matrix metalloproteinases (MMPs) stands among the primary risk factors for the development of cardiopathies. This study aims to optimize a zymogram protocol for detecting the protein and metalloproteinase profiles expressed in different evolutionary forms of the T. cruzi parasite. Additionally, it seeks to investigate alterations in the expression of these proteins in the major organs affected during the early and late acute phases of the disease using the Y strain of T. cruzi in a murine model with BALB/c mice. Initially, axenic cultivation of T. cruzi epimastigotes was performed, and healthy mice (control group) were euthanized. The heart, spleen, liver, and blood were collected for total extract and blood serum production. SDS-PAGE and zymogram techniques were employed to assess the protein and proteolytic profile of expressed metalloproteinases. The zymogram, using a 10% polyacrylamide gel with gelatin substrate, facilitated the determination of the metalloproteinase profile in healthy mice, identifying several proteins (gelatinases) with molecular weights similar to or equal to those described in the literature. The metalloproteinase profile of T. cruzi epimastigotes was also determined, revealing bands of proteolytic activity at approximate molecular weights of 93 kDa and 26 kDa, with a zone of weak activity dispersed between 65 and 26 kDa. Subsequent experiments will produce and discuss extracts from infected mice and metacyclic trypomastigotes concerning potential modifications in protein expression, considering relevant literature. Thus, the goal is to assess how T. cruzi infection interferes with the expression of metalloproteinases normally expressed in non-infected tissues during the early and late acute phases of Chagas disease.

COMMITTEE MEMBERS:
Externa à Instituição - Sílvia Donato - UFCG
Externo à Instituição - TAFFAREL MELO TORRES - UFERSA
Presidente - 2213126 - VALTER FERREIRA DE ANDRADE NETO