Banca de DEFESA: FABIANA MARIA COIMBRA DE CARVALHO

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : FABIANA MARIA COIMBRA DE CARVALHO
DATE: 14/12/2019
TIME: 08:00
LOCAL: Auditório da Superintendência de Infraestrutura
TITLE:

Evaluation of the effect of purified tamarind seed trypsin inhibitor on metabolic changes induced by experimental diet in a wistar rat model.

KEY WORDS:

Tamarindus indica L .. Lipid profile. Blood glucose. PPAR- γ. TNF-α.

PAGES: 90
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUMMARY:

The increasing prevalence of obesity and all associated noncommunicable chronic diseases (NCDs) has increased the search for new methods to combat them. To investigate the effect of the nutritional pathways of nutritionally unbalanced diets on markers and/or parameters metabolic/biochemical, inflammatory and molecular, and look for molecules that can act by reverse this situation is essential. In this study, an experimental diet was produced and its centesimal composition, glycemic index and glycemic load were analyzed. For glycemic index and glycemic load analyzes two groups of Wistar rats were used. It was also evaluated the metabolic changes induced by either this experimental diet and the tamarind seed purified trypsin inhibitor (ITTp). The experiments were conducted in two phases: 1) 17 weeks, in which two groups of Wistar rats (n = 5) were used, one group of animals were fed with the experimental diet, while the other group with the standard diet (Labina®). At the end of this phase, were analyzed: nutritional status, andbiochemical, inflammatory, hormonal and molecular parameters and markers. 2) four groups of Wistar rats (n = 5) were used. Of these, three groups of animals were initially fed for 17 weeks with an experimental diet and presented obesity and then two of these groups were treated for 10 days with a) standard diet or b) ITTp (730 µg / kg). The third group of obese Wistar rats received no treatment (negative control) and one more group of eutrophic Wistar rats were added to the experiment as a positive control. On the 11th day of the experiment were evaluated: zoomometric measurements; consumption and weight gain; and biochemical and molecular parameters and markers. The experimental diet showed high glycemic index (77.6) and high glycemic load (38.8). There was a statistically significant difference (p <0.0001) in the cumulative weekly difference in Lee Index values, evidencing an increase in the 17 weeks of study in the group that received the experimental diet. The animals that received the experimental diet presented significantly increased fasting glucose (p = 0.008) when compared to the animals fed by standard diet. There were also statistically significant difference in relation to VLDL-c (p = 0.016) and TG (p = 0.016), being higher in the group of animals that received the experimental diet. Rats from the experimental diet group had significantly higher PPARγ mRNA relative expression in the gonadal and retroperitoneal tissues (p = 0.0121 and p = 0.0024, respectively). Regarding the effect of pTTI on food intake, the pTTI-treated groupshowed significantly lower medians thanthe experimental diet group (p = 0.0065). About the effect of ITTp on biochemical variables, the group of obese Wistar rats treated with ITTp had the lowest TG and VLDL-c concentrations (p = 0.0108) compared to the other groups. In addition, the group of animals treated with ITTp had significantly lower TNF-α mRNA relative expression (p = 0.025) compared to the other groups, regardless of PPAR-γ mRNA relative expression. This present study presents the ITTp as a bioactive molecule that acts on the modulation of metabolic changes caused by a high glycemic index experimental diet. Therefore, ITTp  is a molecule with great biotechnological potential, especially for the development of biopharmaceuticals with anti-TNF-α function.

BANKING MEMBERS:
Externa ao Programa - 2323511 - ADRIANA AUGUSTO DE REZENDE
Presidente - 2578619 - ANA HELONEIDA DE ARAUJO MORAIS
Externa ao Programa - 349432 - LUCIA DE FATIMA CAMPOS PEDROSA
Externa à Instituição - MAIRA CONCEIÇÃO JERONIMO DE SOUZA LIMA - UnP
Externa à Instituição - PAULA IVANI MEDEIROS DOS SANTOS - IFRN