Banca de QUALIFICAÇÃO: YAGO QUEIROZ DOS SANTOS

Uma banca de QUALIFICAÇÃO DE MESTRADO foi cadastrada pelo programa.

DISCENTE: YAGO QUEIROZ DOS SANTOS
DATA: 19/05/2016
HORA: 14:00
LOCAL: Sala de reuniões do CB
TÍTULO:

Production, purification and characterization of a salt-tolerant thermostable cellulase from a marine Bacillus sp. strain

RESUMO:

A salt-tolerant cellulase secreted by a marine Bacillus sp. SR22 strain with wide resistance to temperature and pH was isolated and characterized. The 37.35 kDa endoglucanase named as Bc22Cel was purified by ammonium sulphate precipitation, gel filtration chromatography and extraction from the gel after non-reducing sodium dodecylsufate-polyacrylamide gel electrophoresis. The optimal pH value and temperature of Bc22Cel were 6.5 and 60 °C, respectively. The purified Bc22Cel showed a considerable halophilic property being able to maintain more than 70% of residual activity even when pre-incubated with 1.5 M NaCl for 1 hour. Kinetic analysis of purified enzyme showed the Km and Vmax to be 0,3953 nmol ml-1 and 0,0167 μmol ml-1 min-1, respectively. Taking together, the present data indicates the Bc22Cel as a potential and useful candidate for industrial applications.

PALAVRAS-CHAVE:

Sugarcane bagasse-degrading bacteria,Thermostable enzymes, Bioprocessing

PÁGINAS: 13
GRANDE ÁREA: Ciências Biológicas
ÁREA: Bioquímica

MEMBROS DA BANCA:
Presidente - 1880243 - DANIEL CARLOS FERREIRA LANZA
Interno - 1544647 - MATHEUS DE FREITAS FERNANDES PEDROSA
Externo à Instituição - PAULA IVANI MEDEIROS DOS SANTOS - IFPI