Banca de QUALIFICAÇÃO: MARCOS FELIPE DE OLIVEIRA GALVAO

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
DISCENTE: MARCOS FELIPE DE OLIVEIRA GALVAO
DATA: 15/03/2016
HORA: 13:30
LOCAL: Sala Carl Peter von Dietrich - Depto. de Bioquímica - CB/UFRN
TÍTULO:

PAH exposure and relationship between buccal micronucleus cytome assay and urinary 1-hydroxypyrene levels among cashew nut roasting workers

PALAVRAS-CHAVES:

Cashew nut roasting, Particulate matter, Human biomonitoring, Occupational exposure, 1-Hydroxypyrene, Buccal micronucleus cytome assay (BMCyt)

PÁGINAS: 37
GRANDE ÁREA: Ciências Biológicas
ÁREA: Bioquímica
SUBÁREA: Biologia Molecular
RESUMO:

The present study conducted the first assessment of the occupational risk associated to artisanal cashew nut roasting by the use of exposure and effect biomarkers, as well as the characterization and dispersion analysis of the released particulate matter (PM). A real-time airborne particle monitor was used to quantify PM_1.0, PM_2.5 and PM₁₀ concentrations. Furthermore, the PM was sampled using a Handi-vol sampler and the physicochemical characteristics were determined by gravimetric and SEM-EDS analysis. Trajectories, dispersion and deposition of the emitted material were calculated using the NOAA-HYSPLIT model. Urinary 1-hydroxypyrene (1-OHP) levels were analyzed by high-performance liquid chromatography. DNA damage, chromosomal instability and cell death were measured by the buccal micronucleus cytome assay (BMCyt assay). The PM concentration for all measurements in the exposed area was higher than in the non-exposed area (p < 0.0001). Furthermore, it was observed that the control area yielded a higher prevalence of coarse particles, while a higher prevalence of fine particles was observed in the exposed area. The SEM-EDS analyses showed a wide variety of irregular particles in the samples, such as tar balls, smooth-surfaced and mineral particles. Biomass burning tracers K, Cl, S and Ca were the major inorganic compounds found. The dispersion analysis suggested that the PM_2.5 can reach neighboring regions in a distance to the order of 40 kilometers. PAH exposure was confirmed by increases in urinary 1-OHP levels in cashew nut workers. The frequencies of BMCyt biomarkers were higher in the exposed group (p < 0.0001) than in the control group. The influence of factors such as age range and smoking status on the micronucleus (MN) frequency was evidenced and a correlation (r = 0.64; p < 0.0001) between the exposure (1-OHP) and effect (MN) biomarker was observed. The physico-chemical and dispersion analyses of the PM showed typical particles from biomass burning with the potential to reach neighboring regions. The PAH exposure and genotoxic potential among cashew nut workers were confirmed by the increase in urinary 1-OHP levels and BMCyt biomarkers and it was the first study to found a positive correlation between these type of biomarkers. The uses of exposure and effect biomarkers were therefore efficient in assessing the occupational risk associated with artisanal cashew nut roasting. The high rates of PM_2.5 are considered a potential contributor to this effect.

MEMBROS DA BANCA:
Externo à Instituição - GLAUBER JOSE TUROLLA FERNANDES - NENHUMA
Externo ao Programa - 1879213 - JUDITH JOHANNA HOELZEMANN
Presidente - 032.540.854-85 - TIRZAH BRAZ PETTA LAJUS - UFRGS