Banca de QUALIFICAÇÃO: MARINA SAMPAIO DE MENEZES CRUZ

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE: MARINA SAMPAIO DE MENEZES CRUZ
DATA: 07/05/2015
HORA: 14:30
LOCAL: Anfiteatro dos Peixes - Centro de Biociências
TÍTULO:

Diosgenin induce genotoxic and mutagenic effect on HepG2 cells


PALAVRAS-CHAVES:

Diosgenin, cytotoxicity, genotoxicity, mutagenicity


PÁGINAS: 27
GRANDE ÁREA: Ciências Biológicas
ÁREA: Bioquímica
RESUMO:

In the present study the cytotoxic, genotoxic and mutagenic potential of Diosgenin on HepG2 cells were investigated. Cytotoxicity was assessed using MTT and clonogenic assay. Besides, genotoxic and mutagenic effect were performed using Single cell gel electrophoresis and Cytokinesis-block micronucleus assay, respectively. A reduction on cell viability and cell replication was observed due to Diosgenin treatment at concentrations higher than 30uM and 40uM, respectively. In addition, a statistic genotoxic effect was observed by Comet assay at concentrations from 40uM. CBMN revealed a significant increased in micronucleus frequency along with a significant cytostatic effect in the same range of concentrations. Diosgenin caused DNA damage on Hep2G cells. This effect could not be efficiently repaired contributing to a development of mutagenic effect. These facts showed that Diosgenin deleterious effect could take place through genetic instability fact that affect the normal cell cycle leading to trigger the apoptotic mechanism.


MEMBROS DA BANCA:
Presidente - 2195251 - HUGO ALEXANDRE DE OLIVEIRA ROCHA
Externo ao Programa - 2085604 - SUSANA MARGARIDA GOMES MOREIRA
Externo à Instituição - JULIO ALEJANDRO NAVONI - NENHUMA
Notícia cadastrada em: 05/05/2015 10:09
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