Diosgenin induce genotoxic and mutagenic effect on HepG2 cells
Diosgenin, cytotoxicity, genotoxicity, mutagenicity
In the present study the cytotoxic, genotoxic and mutagenic potential of Diosgenin on HepG2 cells were investigated. Cytotoxicity was assessed using MTT and clonogenic assay. Besides, genotoxic and mutagenic effect were performed using Single cell gel electrophoresis and Cytokinesis-block micronucleus assay, respectively. A reduction on cell viability and cell replication was observed due to Diosgenin treatment at concentrations higher than 30uM and 40uM, respectively. In addition, a statistic genotoxic effect was observed by Comet assay at concentrations from 40uM. CBMN revealed a significant increased in micronucleus frequency along with a significant cytostatic effect in the same range of concentrations. Diosgenin caused DNA damage on Hep2G cells. This effect could not be efficiently repaired contributing to a development of mutagenic effect. These facts showed that Diosgenin deleterious effect could take place through genetic instability fact that affect the normal cell cycle leading to trigger the apoptotic mechanism.