Banca de DEFESA: PRISCILA CAROLINE DE SOUSA COSTA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : PRISCILA CAROLINE DE SOUSA COSTA
DATE: 15/12/2022
TIME: 09:00
LOCAL: Google Meet
TITLE:

IDENTIFICATION OF REMOTE HOMOLOGUES USING PROTEIN STRUCTURAL ALIGNMENT AND MACHINE LEARNING TOOLS

KEY WORDS:

DUF, protein domains, AlphaFold, structural similarity of proteins, FATCAT

PAGES: 37
BIG AREA: Ciências Biológicas
AREA: Biologia Geral
SUMMARY:

Proteomics studies have shown the large number of proteins discovered and their importance to the study of life. However, there is still a high percentage of these proteins that have not been functionally annotated, limiting advances in several areas with healthcare and biotechnology. The functions of proteins are defined by their conformation and changes in the protein's three-dimensional structure, so data on the three-dimensional structure of these proteins helps in defining their functions. Currently, there is a large amount and diversity of proteins that have their sequence characterized, however, there is still a methodological bottleneck in obtaining their structural data. With the recent development of the AlphaFold program, which accurately predicts the three-dimensional structure of proteins from their amino acid sequence, this bottleneck can be overcome. Thus, the goal of this project is to evaluate the impact of using these structural prediction tools on functional annotations of proteins. In this work, we aim to functionally annotate protein domains of unknown function (DUF). To this end, predicted data of their three-dimensional structure was submitted to computational tools that perform a search for other structures that share structural similarity. Preliminary analyses have shown that many domains can benefit from this analysis. In addition, we generated a classification model that identifies whether two proteins that share a structural similarity are remote homologs. This classifier will be used in the future to analyze the similarity results and suggest functions to these domains.

COMMITTEE MEMBERS:
Externo à Instituição - LUCAS BLEICHER - UFMG
Externo ao Programa - 3884005 - PATRICK CESAR ALVES TERREMATTE - nullPresidente - 3063244 - TETSU SAKAMOTO