Banca de QUALIFICAÇÃO: CARINA IONÁ DE OLIVEIRA TORRES

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : CARINA IONÁ DE OLIVEIRA TORRES
DATE: 28/02/2023
TIME: 10:00
LOCAL: Auditório do Museu de Ciências Morfológicas
TITLE:

ANIMAL MODEL OF DEPRESSION FOR DRUG STUDY FAST-ACTING ANTIDEPRESSANTS


KEY WORDS:

Fast-acting antidepressants; Chronic corticosterone exposure; Rodent model; Major depressive disorder.


PAGES: 52
BIG AREA: Ciências Biológicas
AREA: Morfologia
SUMMARY:

Major Depressive Disorder (MDD) is characterized as one of the emotional disorders most prevalent and disabling in the world. However, pharmacological treatment of depression has important features, such as low strength, presence of several side effects and the delay in promoting therapeutic effects, which lead patients to abandon treatment.Thus, the identification of drugs fast-acting antidepressants is necessary and, for that, animal models are useful tools, bearing in mind the ethical and practical limitations linked to studies in humans. However, there are still no models of depression capable of detecting selectively rapid-acting antidepressants and the development of an animal model being able to select drugs with this profile could represent a substantial advance in the treatment and understanding of MDD and its biological bases. In that sense, the This project has the general objective of evaluating chronic exposure to corticosterone orally as an animal model to identify fast-acting antidepressants. For such, male Swiss mice were submitted to pilot assays to select the best type of corticosterone and the appropriate experimental conditions for carrying out of behavioral tests. Then, the animals were divided into two groups and received the following treatments for 42 and 28 uninterrupted days: mineral water (control) and corticosterone (hydrocortisone dissolved in water 0.1 and 0.03 mg/ml). To the throughout the exposure period, sucrose preference tests were performed, open field, marble buring test, tail suspension, forced swimming and interaction to investigate behaviors associated with depression, anxiety, and locomotor activity. On the 21st day, mice treated with hydrocortisone were divided into groups that received ketamine (10 and 100 mg/kg, ip) or vehicle. You The results obtained suggest that hydrocortisone 0.1 mg/ml was able to induce behaviors related to depression and anxiety, in addition to hyperlocomotion in animals, but associated with great weight loss and death. At a lower dose, the hydrocortisone caused an anxiogenic effect in the first days of exposure, while the anhedonic behavior appeared after 2 weeks of exposure to corticosteroid. Despite the small sample size, acute treatment with ketamine (the depending on the dose), in turn, tended to reverse the anhedonia, the anxiogenic effect and hyperlocomotor caused by hydrocortisone 0.03 mg/ml, mainly after 4 days of its administration. Finally, the data presented here indicate that the proposed model repeated exposure to oral hydrocortisone was able to mimic the changes behavioral (symptomological) symptoms presented by patients with MDD, thus having a good face validation.


COMMITTEE MEMBERS:
Presidente - 1645202 - ELAINE CRISTINA GAVIOLI
Interna - 1720860 - VANESSA DE PAULA SOARES RACHETTI
Externa ao Programa - 3274279 - VINICIA GARZELLA METZ - null
Notícia cadastrada em: 27/02/2023 15:02
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