Banca de DEFESA: VICTOR MORAES RODRIGUES
Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.STUDENT : VICTOR MORAES RODRIGUES
DATE: 06/03/2023
TIME: 09:30
LOCAL: REMOTA - https://meet.google.com/yzy-xgfc-wwe
TITLE:
STUDY OF PRODUCTION, CHARACTERIZATION AND IN VITRO BIOCOMPATIBILITY OF POLYMERIC NANOCAPSULES CONTAINING COPAIBA OIL IN LUNG CELLS
KEY WORDS:
copaiba oil; polymeric nanocapsules; cytotoxicity; genotoxicity;
PAGES: 52
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:
Copaiba oil has been largely used due to its therapeutic properties. Nanocapsules were revealed to be a great nanosystem to carry natural oils due to their ability to improve the bioaccessibility and the bioavailability of lipophilic compounds. The aim of this study was to produce and characterize copaiba oil nanocapsules (CopNc) and to evaluate their hemocompatibility, cytotoxicity, and genotoxicity. Copaiba oil was chemically characterized by GC-MS and FTIR. CopNc was produced using the nanoprecipitation method. The physicochemical stability, toxicity, and biocompatibility of the systems, in vitro, were then evaluated. β-bisabolene, cis-α-bergamotene, caryophyllene, and caryophyllene oxide were identified as the major copaiba oil components. CopNc showed a particle size of 215 ± 10 nm, a polydispersity index of 0.15 ± 0.01, and a zeta potential of −18 ± 1. These parameters remained unchanged over 30 days at 25 ± 2 °C. The encapsulation efficiency of CopNc was 54 ± 2%. CopNc neither induced hemolysis in erythrocytes, nor cytotoxic and genotoxic effects in lung cells at the range of concentrations from 50 to 200 μg·mL−1. In conclusion, CopNc showed suitable stability and physicochemical properties. Moreover, this formulation presented a remarkable safety profile on lung cells. These results may pave the way to further use CopNc for the development of phytotherapeutic medicine intended for pulmonary delivery of copaiba oil.
COMMITTEE MEMBERS:
Presidente - 1178187 - ERYVALDO SOCRATES TABOSA DO EGITO
Externa ao Programa - 2374605 - AURIGENA ANTUNES DE ARAUJO - UFRNExterno à Instituição - FRANCISCO HUMBERTO XAVIER JUNIOR - UFPB
Externo à Instituição - LUCAS AMARAL MACHADO