Banca de QUALIFICAÇÃO: ONY ARAÚJO GALDINO

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : ONY ARAÚJO GALDINO
DATE: 24/02/2023
TIME: 08:00
LOCAL: DEFESA REMOTA - https://meet.google.com/vmz-nvie-gvm
TITLE:

PASSIFLORA EDULIS AS ADJUVANT THERAPY FOR THE TREATMENT OF DIABETIC KIDNEY DISEASE: ASSESSMENT OF EARLY BIOMARKERS OF KIDNEY INJURY

KEY WORDS:

Diabetic Kidney Disease; Adjuvant Therapy; Biomarkers

PAGES: 20
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Diabetic kidney disease (DKD) is a frequent and serious microvascular complication in type 1 diabetes (T1D), resulting from damage to the glomeruli and renal tubules, being mediated by morphological changes in the renal tissue. These changes result in the loss of podocyte and tubular proteins into the urinary space, such as nephrin, podocin, WT1, and megalin. Considering that adequate glycemic control (GC) in T1D can prevent DKD and that insulin associated with adjuvant therapies favors a good GC, Passiflora edulis (P. edulis) fruit peel extracts should represent a potential hypoglycemic agent that may prevent and/or reduce kidney damage associated with DKD. This study aims to evaluate the adjuvant therapeutic effect of P. edulis fruit peel extract in the prevention of kidney damage caused by DKD in streptozotocin-induced diabetic rats. Male Wistar rats were distributed in 4 groups according to the treatment received for 60 days: control (C); diabetic (D); insulin-treated diabetic (IN); diabetic treated with insulin and aqueous extract of P. edulis 400 mg/kg (IN+AFA). Nephrin, podocin, WT1, and megalin were measured by Western blot from urinary extracellular vesicles and kidney tissue. Nphs1 (nephrin), nphs2 (podocin), WT1, and Lrp2 (megalin) mRNA expressions were evaluated in kidney tissue by RT-qPCR. Serum and urine biochemical analyses were evaluated. It was observed that rats treated with IN+AFA had a significant reduction in serum glucose compared to those treated with isolated insulin (IN) and untreated (D), showing that good CG is provided by P. edulis in association with insulin. This CG reflected in protection against kidney damage since the protein expression of nephrin, podocin, WT1, and megalin in kidney tissue was significantly higher in IN+AFA rats when compared to D, demonstrating that the combined therapy of P. edulis influences conservation of the kidney tissue. Therefore, this pioneering research showed the potential of the adjuvant hypoglycemic effect of P. edulis in reducing kidney damage and consequently preserving renal glomerular and tubular constituents.

BANKING MEMBERS:
Presidente - 2323511 - ADRIANA AUGUSTO DE REZENDE
Interno - 1451614 - JOSIVAN GOMES DE LIMA
Externa ao Programa - 1055045 - MARCELA ABBOTT GALVAO URURAHY