Banca de QUALIFICAÇÃO: VINÍCIUS BARRETO GARCIA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : VINÍCIUS BARRETO GARCIA
DATE: 09/11/2021
TIME: 09:30
LOCAL: DEFESA REMOTA - https://meet.google.com/awb-nkbf-pve
TITLE:

ENVIRONMENTALLY COMPATIBLE BIOCONJUGATED GOLD NANOPARTICLES AS EFFICIENT CONTRAST AGENTS FOR INFLAMMATION-INDUCED CANCER IMAGING

KEY WORDS:

Golden nanoparticles, M2 macrophages, Immunofluorescence, Inflammatory processes, Cancer diagnostics, Ulcerative colitis

PAGES: 12
BIG AREA: Ciências da Saúde
AREA: Medicina
SUMMARY:

For many cancers, early detection is the key to improve survival and reduce the morbidity, which is associated with radical resections due to late diagnosis. Here, we describe the efficiency of primary antibody-conjugated gold nanoparticles (AuNPs) to specifically target chronic inflammatory processes, specially M2 macrophages, in tissue sections of ulcerative colitis (UC) and steatohepatitis in rats which may lead to colorectal cancer and liver carcinoma, respectively. In this study, we demonstrate that AuNPs synthesized by a simple, inexpensive, and environmentally compatible method can be easily conjugated with the antibodies anti-COX-2, anti-MIF, and Alexa Fluor® 488 (ALEXA) to perform immunofluorescence staining in inflamed tissues. Moreover, we showed that primary antibody-conjugated gold nanoparticles (AuNPs) can be used to target M2 macrophages by flow cytometry. We designed three immunofluorescence staining protocols of tissue section with AuNPs for 30 min and overnight incubation, as well as one flow cytometry protocol of M2 macrophage labeling with AuNPs for 30min. Immunofluorescence and flow cytometry results suggest that conjugation was achieved by direct adsorption of antibodies on the AuNPs surface. When compared to the standard ALEXA protocol in immunofluorescence (IF) and flow cytometry (FC), our 30-min incubation protocol using AuNPs instead of ALEXA decreased from approximately 23h to 5h for IF and from 4h to 1h for FC, proving to be less laborious, which makes the method eligible for inflammation-induced cancer diagnostic.

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