Banca de QUALIFICAÇÃO: EDILANE RODRIGUES DANTAS DE ARAÚJO

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : EDILANE RODRIGUES DANTAS DE ARAÚJO
DATE: 30/08/2021
TIME: 14:00
LOCAL: DEFESA REMOTA - meet.google.com/kro-muai-hhx
TITLE:

GASTRIC ULCER HEALING PROPERTY OF BRYOPHYLLUM PINNATUM LEAF EXTRACT IN CHRONIC MODEL IN VIVO AND GASTROPROTECTIVE ACTIVITY OF ITS MAJOR FLAVONOID.

KEY WORDS:

Gastric ulcer; ulcer healing; gastroprotection; Bryophyllum pinnatum; flavonoids; quercetin.

PAGES: 42
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Gastric ulcer is a common disease that when not properly treated is able to develop complications such as hemorrhages and perforations. Extended use of drugs in treatment this pathology can provoke many adverse effects. Therefore, the investigation of medicinal plants with gastroprotective and mucosal healing properties has gained increasing interest. Bryophyllum pinnatum (Crassulaceae), popularly known as "saião" or "coirama" has been used to treat inflammatory disorders, this specie is rich in flavonoids and quercetin 3-O-α-L-arabinopyranosyl-(1à2)-O-α-L-rhamnopyranoside is the major compound. In this context, the aim of this study is to evaluate ulcer healing property of the B. pinnatum against acetic acid-induced chronic ulcer model and the gastroprotective activity of the major flavonoid against gastric lesions induced by ethanol and indomethacin. Ultra-fast liquid chromatography method used for the quantification of the main compounds indicated good linearity, specificity, selectivity, precision, robustness and accuracy. HPLC results quantified the flavonoids (mg/g of the extract) quercetin 3-O-α-L-arabinopyranosyl-(1à2)-O-α-L-rhamnopyranoside (33.12 ± 0.056), kaempferol 3-O-α-L-arabinopyranosyl-(1à2)-O-α-L-rhamnopyranoside (3.98 ± 0.049) and kaempferol 3-O-α-L-rhamnopyranoside (4.26 ± 0.022). The in vivo studies shows that the treatment with the extract at doses of 250 and 500 mg/kg stimulated the healing process in the gastric mucosa with a significant ulceration index reduction followed by improvement of antioxidant defense system with a glutathione increased levels, superoxide dismutase upregulation and malondialdehyde decreased levels. Moreover, the extract decreased interleukin-1β and Tumor Necrosis Factor-a levels and myeloperoxidase activity, increased interleukin 10 levels, showed a cytoprotective effect in histological analyses and also downregulated the expression of COX-2 and NF-κB (p65). The pre-treatment with major flavonoid at dose of 5 mg/kg reduced the gastric lesions in ethanol and indomethacin models, increased glutathione levels and decreased malondialdehyde levels. In addition, the pre-treatment was able to decrease myeloperoxidase activity, interleukin-1β and tumor necrosis factor-α levels, while also showing a cytoprotective effect in histological analyses. In this study was observed that the treatment with B. pinnatum extract showed a higher inhibition percentage when compared to pre-treatment with the major flavonoid. This might suggest that major flavonoid has gastroprotective activity but other substances present in B. pinnatum leaf can act synergistically, potentiating the anti-inflammatory and, consequently, anti-ulcer activity. In conclusion, these results suggest that B. pinnatum leaf extract can be a new source of raw material rich- phenolics to be applied as food or medicine.

BANKING MEMBERS:
Interno - 2195251 - HUGO ALEXANDRE DE OLIVEIRA ROCHA
Externa ao Programa - 2330188 - GERLANE COELHO BERNARDO GUERRA
Externo à Instituição - RENAN OLIVEIRA SILVA DAMASCENO - UFPE