Banca de DEFESA: ADRIANA OLIVEIRA DE SOUZA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ADRIANA OLIVEIRA DE SOUZA
DATE: 18/12/2020
TIME: 14:00
LOCAL: DEFESA REMOTA - https://meet.google.com/rdx-hyxg-uvm
TITLE:

ANTI-TRYPANOSOMA CRUZI SILVER NANOPARTICLES CONTAINING FUCAN FROM SEAWEED SPATOGLOSSUM SCHRÖEDERI: SYNTHESIS AND CHARACTERIZATION


KEY WORDS:

Fucoidan; Chagas disease; Brown seaweed; anti-Trypanosoma cruzi activity


PAGES: 16
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUMMARY:

The brown seaweed Spatoglossum schröederi synthesizes three types of bioactive heterofucans, the most abundant of which is fucan A. Silver nanoparticles containing fucan A (AgFuc) were produced using an environmentally friendly method. Fucan was extracted from S. schröederi and its identity was confirmed by chemical analysis, Fourier transform infrared spectroscopy (FTIR) and agarose gel electrophoresis. The synthesis of AgFuc was analyzed by UV-vis spectroscopy at kmax = 440 nm and FTIR, which confirmed the presence of silver and fucan A in the product AgFuc. Dynamic light scattering (DLS), X-ray diffraction, scanning electron microscopy and atomic force microscopy revealed that the AgFuc particles were approximately 180.0 nm in size and spherical shape. DLS also produced that AgFuc was stable for 5 months and optical coupled plasma emission spectrometry (ICP-OES) showed that the AgFuc particles contained 5% silver. AgFuc can also be more effective in inhibiting the parasites' ability to reduce MTT than fucan A or silver, regardless of treatment time. In addition, AgFuc induced the death of ~ 60% of the parasites by necrosis and ~ 17% by apoptosis. Therefore, AgFuc induces damage to the parasites' mitochondria, which must be an anti-Trypanosoma cruzi agent. This is the first study to analyze silver nanoparticles containing fucana as an anti-Trypanosoma cruzi agent. Our data indicate that AgFuc nanoparticles have therapeutic applications, which must be determined through pre-clinical studies in vitro and in vivo


BANKING MEMBERS:
Presidente - 2195251 - HUGO ALEXANDRE DE OLIVEIRA ROCHA
Externo ao Programa - 1752367 - PAULO MARCOS DA MATTA GUEDES
Externa à Instituição - VALQUIRIA PEREIRA DE MEDEIROS
Notícia cadastrada em: 06/12/2020 23:59
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