Banca de DEFESA: ARTHUR SÉRGIO AVELINO DE MEDEIROS

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : ARTHUR SÉRGIO AVELINO DE MEDEIROS
DATE: 18/11/2020
TIME: 14:00
LOCAL: DEFESA REMOTA - https://meet.google.com/upz-axey-tjz
TITLE:

DEVELOPMENT OF CATIONIC NANOEMULSION FOR VENOM-LOAD OF TITYUS SERRULATUS SCORPION AND EVALUATION OF EFFICACY FROM SERUM PRODUCED BY IMMUNIZED MICE


KEY WORDS:

Scorpion antivenom; nanotechnology; nanoemulsion adjuvant; liquid crystal; phase transition; surface functionalization; immunoadjuvant.


PAGES: 68
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Scorpion stings are major cause of envenoming by animals for endemic countries, like Brazil. The antivenom is a neutralizing drug to protect susceptible people, but its production is subject of improvements, like adjuvant innovations and the difficulty of venom extraction from captive animals and its toxicity. Scorpion venom has a complex composition and an adjuvant for crude venom presents a challenge that can be possibly fulfilled with drug delivery systems based on nanotechnology. In this research, we seek for innovation developing new nanoemulsion formulations to act as drug delivery system for Tityus serrulatus scorpion venom and to evaluate immunizing Balb/C mice. The nanotechnology development covered features that positively affected adjuvant activity. We demonstrated that low energy techniques for obtainment nanoemulsions allowed long term stability to the formulations with reduced and uniform droplet size (below 200 nm). The developed formulations were also robust to pH and saline adjustment and to cationic covering with poly(ethyleneimine) (PEI) maintaining stability. The formulations demonstrated capable to nonspecifically adsorb crude venom, as showed through FTIR spectroscopy technique as intermolecular interactions between nanoemulsions and crude venom. Nanoemulsions venom-load also could reduce toxicity related to venom hemolytic activity and to present low toxicity against macrophage cells (RAW 264.7). Adjuvant activity was demonstrated in comparison with aluminum hydroxide adjuvant, which scored for nanotechnology feature of act as crude venom adjuvant more efficiently to induce specific anti-IgG immunoglobulins using PEI-covered nanoemulsions.


BANKING MEMBERS:
Interno - 1639820 - ARNOBIO ANTONIO DA SILVA JUNIOR
Externo ao Programa - 1178187 - ERYVALDO SOCRATES TABOSA DO EGITO
Externo ao Programa - 1544647 - MATHEUS DE FREITAS FERNANDES PEDROSA
Externa à Instituição - CAROLINA ALOISIO - UNC/AR
Externo à Instituição - ANSELMO GOMES DE OLIVEIRA - UNESP
Notícia cadastrada em: 12/11/2020 09:25
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