Banca de DEFESA: DIANA LUZIA ZUZA ALVES SILVA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : DIANA LUZIA ZUZA ALVES SILVA
DATE: 31/03/2020
TIME: 09:00
LOCAL: Sala do CONSEC (2º andar do CCS)
TITLE:

MOLECULAR IDENTIFICATION, GENOTYPING AND GENE EXPRESSION OF CANDIDA TROPICALIS ENVIRONMENTAL AND CLINICAL ISOLATES, WITH EMPHASIS ON VIRULENCE FACTORS AND CELL STRESS

KEY WORDS:

Candida tropicalis, osmotic stress, genotyping, gene expression, virulence

PAGES: 31
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Candida tropicalis is one of the main Candida species, being considered the second most virulent of the genus. Virulence factors related to this species include strong biofilm production, adhesion to human oral epithelial cells, lytic enzyme secretion, morphogenesis and phenotypic switching. In addition to all these characteristics, C. tropicalis is described as an osmotolerant microorganism and this ability to survive in high salt concentrations may be important for fungal persistence in saline environments. This yeast is closely related to C. albicans and is described as the second or third most common etiological agent of candidemia, especially in Latin America and Asia. This fact, combined with the increasingly reported antifungal resistance, demonstrates the need for a better understanding of the genetic variability patterns of this important Candida species in the tropics. Therefore, we made an update of C. tropicalis, focusing on all the subjects mentioned, as well as evaluating the genotypic and phenotypic variability of 62 isolates of C. tropicalis obtained from the coastal environment of Northeast Brazil, using microsatellite and MALDI-TOF / MS. There was a relatively low correspondence between these two typing techniques, so further studies are needed to consolidate the use of MALDI-TOF / MS as a yeast typing tool. However, both methods showed heterogeneity of C. tropicalis in the coastal environment. We also found relative maintenance of population structure in the same climate season, which may reinforce the idea that this species has the potential to remain in the environment for a long period of time. In addition, highly related strains were found at different geographic collection points, demonstrating that this species can disperse over long distances, probably influenced by anthropogenic actions and driven by tides and wind.

BANKING MEMBERS:
Interno - 346138 - ALDO DA CUNHA MEDEIROS
Interna - 1199080 - ANA KATHERINE DA SILVEIRA GONCALVES DE OLIVEIRA
Presidente - 1715308 - GUILHERME MARANHAO CHAVES
Externa à Instituição - RAQUEL DE MELO BARBOSA - F.M.Nassau
Externo à Instituição - REGINALDO GONÇALVES DE LIMA NETO - UFPE