Banca de QUALIFICAÇÃO: RENATO CESAR DE AZEVEDO RIBEIRO

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
DISCENTE : RENATO CESAR DE AZEVEDO RIBEIRO
DATA : 11/10/2019
HORA: 09:00
LOCAL: sala de aula do PPGCSa
TÍTULO:

Poly(malic acid) nanoconjugates as a controlled release system for the
treatment of inflammatory diseases

PALAVRAS-CHAVES:

(Poly)malic acid, Dexamethasone, Prednisolone, Nanoconjugates,
Antiinflamatory

PÁGINAS: 18
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
RESUMO:

Corticosteroids have an important role on the treatment of inflammatory diseases.
However, these drugs cause many toxic effects that could be avoided using a
controlled release system. Poly(malic acid) (PMA), a biodegradable, non-toxic and
non-immunogenic polyester, has been proven to be a promising carrier for poorly
soluble drugs. The large number of carboxyl groups on its backbone makes this
polymer of particular interest on drug delivery mainly because of the ability to
generate a high drug loading content. In this study, PMA nanoconjugates were
designed aiming to improve the administration of dexamethasone (DXM) and
Prednisolone (PRD) at the inflammatory sites and to achieve increased therapeutic
efficacy and reduced adverse effects. PMA was first synthesized by direct polycondensation
of L-malic acid. Then, covalent conjugation in different proportions of
DXM or PRD with PMA afforded amphiphilic copolymers that readily self-assembled
to form nanoconjugates. These resulting spherical structures showed uniform
distributions of size from 120 to 150 nm and a negative zeta potential from -55 to -60
mV according the drug content. Due to the hydrolytic sensitive linkages between
DXM or PRD and PMA, a gradual and complete drug release was achieved in 2
days. Finally, MTT test revealed no cytotoxicity of the system, up to 30 µg/mL.
Additionally, the anti-inflammatory effect was demonstrated by inhibition of IL-6, IL10
and TNF-α. Also, the internalization studies of the nanoconjugates revealed that it
reached a maximum in 6 hours on macrophages RAW 264.7. Overall, the results
demonstrate that these nanosystems can be a promising drug carrier to be used on
inflammatory conditions.

MEMBROS DA BANCA:
Interno - 346138 - ALDO DA CUNHA MEDEIROS
Presidente - 1178187 - ERYVALDO SOCRATES TABOSA DO EGITO
Externo ao Programa - 1544647 - MATHEUS DE FREITAS FERNANDES PEDROSA