Banca de DEFESA: JOVELINA SAMARA FERREIRA ALVES

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : JOVELINA SAMARA FERREIRA ALVES
DATA : 30/08/2020
HORA: 09:00
LOCAL: Maternidade Escola Januário Cicco
TÍTULO:

Obtaining, characterization and evaluation of antidepressant activity of free flavonoid-rich extract of nanoparticles polymers from leaves of Passiflora edulis fo. flavicarpa (yellow passion fruit)

PALAVRAS-CHAVES:

Passifloraceae, herbal medicine, marker, nanotechnology, depression

PÁGINAS: 111
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
RESUMO:

Species of the genus Passiflora sp. have the ability to produce a variety of neuroactive flavonoids and antioxidants,

 which can prevent brain damage caused by disorders depressive. However, due to the low bioavailability, 

clinical application of these assets is still a challenge for pharmaceutical technology. Within this context, 

we highlight the Passiflora edulis fo. flavicarpa (Passifloraceae), popularly known as yellow passion fruit. 

So the objectives of this paper are: i. develop a method of obtaining an extract rich in flavonoids (FRE) 

from the Passiflora edulis fo. flavicarpa; ii. purify and characterize flavonoids from FRE extract; iii. 

develop a FRE extract loaded nanoparticles system; iv. evaluate the biocompatibility and antidepressant 

activity of FRE extract in free and nanoparticulate form. The leaves used in the study were collected at the place 

of cultivation and dried for use in optimization of extraction by surface response methodology. 

FRE extract was used to isolate the substances majority by HPLC-UV-DAD-Prep. Subsequently, 

it was a system of charged cationic nanoparticles of FRE extract produced with polymethyl methacrylate

 polymer (PMMA), which was characterized by size measurement, zeta potential, encapsulation 

efficiency, ATR-FTIR and AFM microscopy. Additionally, the biocompatibility (through acute oral toxicity model)

 and antidepressant effect (using swim models open field) after oral administration of FRE extract

in free and nanoparticulate form and the substance isoorientina, in in vivo models. 

Quantitative analysis showed that the FRE extract has total flavonoid content of 11.42 mg / g 

and a content of 5.21 mg / g isoorientine. 13 identified glycosylated flavonoids derived from luteoline,

 chrysin and apigenin by HPLC-ESI-IT-MS / MS analysis. Subsequently, the content of the flavonoids vicenin-2 

was evaluated. (5.20 mg / g), orientin (0.59 mg / g), vitexin (0.20 mg / g) and isovitexin (0.96 mg / g) in

 the FRE extract by analysis by CLAE-QqQ-MS / MS system. Physicochemical analysis showed that FRE extract

 loaded nanoparticles are spherical with positive surface charge, size 65.6 nm, positive zeta potential and 

high encapsulation efficiency of isoorientin (E.E = 100%) and stable over six months. At histopathological

 and biochemical analyzes of tissues and serum of animals showed the biocompatibility of nanoparticles in

 white, free FRE extract (2 g / kg kg; p.o.) and nanoparticulate (5 mg / kg; p.o.). The antidepressant-like

 effect after administration oral route of free FRE extract (50 and 100 mg / kg; P <0.01 and P <0.001)

 and nanoparticulate (5 mg / kg; P <0.01) and isoorientine (1-10 mg / kg, P <0.001) was investigated 

in the swim test and similar to the nortriptyline antidepressant, promoted a reduction in the immobility

 time of the animals. Thus, the extraction method optimization increased by 10 times the antidepressant 

potential e. encapsulation promoted the maintenance of antidepressant activity with the reduction of

 extract dose in 10 times. Also, this work was the first to demonstrate the antidepressant effect of the 

substance isoorientina, suggesting a marker. The experiments confirm that active pharmaceutical ingredients 

in free and edulis leaf nanoparticles are a strategy promising for herbal medicine production with action 

antidepressant.

MEMBROS DA BANCA:
Externo à Instituição - GEISON MODESTI COSTA
Externo ao Programa - 1789788 - ADLEY ANTONINI NEVES DE LIMA
Externo à Instituição - EDUARDO PEREIRA DE AZEVEDO - UnP
Presidente - 1490222 - SILVANA MARIA ZUCOLOTTO LANGASSNER
Externa ao Programa - 1720860 - VANESSA DE PAULA SOARES RACHETTI