Banca de DEFESA: TALITA KATIANE DE BRITO PINTO

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : TALITA KATIANE DE BRITO PINTO
DATA : 26/08/2020
HORA: 14:00
LOCAL: Sala Carl Peter von Dietrich
TÍTULO:

Trypanosomicidal activity evaluation of silver-formed nanoparticles and the xylan polysaccharide against epimastigote forms of Trypanosoma cruzi

PALAVRAS-CHAVES:

Xylan; Silver nanoparticles; Trypanosoma cruzi; Benzonidazole

PÁGINAS: 72
GRANDE ÁREA: Ciências Biológicas
ÁREA: Bioquímica
RESUMO:

Chagas disease or American trypanosomiasis is caused by the protozoan Trypanosoma cruzi. The current treatment comprises the use of at least two drugs, benzonidazole (Bnz) and nifurtimox, which present high toxicity and inefficiency in the chronic phase of the disease, which induces the search for more effective therapeutic alternatives. Studies have shown that the green synthesis of silver nanoparticles with polysaccharides has potentiated the biological activity of both polysaccharides and silver. Therefore, the xylan polysaccharide was extracted from corn cob using an environmentally friendly method and was used for the synthesis of silver nanoparticles (NX). Atomic force microscopy, infrared and Raman spectroscopy studies, and coupled plasma atomic emission spectrometry have shown the presence of silver (~ 19%) and xylan in NX, as well as their round shape and average size (~ 40 nm). . Parasites T. cruzi lost 95% of their ability to reduce MTT (3-bromide (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium) when incubated with nanoxylan (100 µg / mL). Flow cytometric analysis showed that 98% of the parasites were positive for propidium iodide when incubated for 24 h with NX (100 µg / mL), while Bnz at the same concentration was unable to induce this effect. The data show that nanoxylan induces parasite necrosis and is a strong candidate for preclinical studies with T. cruzi infected animals.

MEMBROS DA BANCA:
Externa à Instituição - ARIANE FERREIRA LACERDA - IFRN
Externa ao Programa - 1718086 - DANIELLE BARBOSA MORAIS
Presidente - 2195251 - HUGO ALEXANDRE DE OLIVEIRA ROCHA
Externa à Instituição - NATHALIA KELLY DE ARAUJO - IFRN
Externo ao Programa - 2213126 - VALTER FERREIRA DE ANDRADE NETO