Banca de DEFESA: LUCAS AMARAL MACHADO

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : LUCAS AMARAL MACHADO
DATA : 04/06/2020
HORA: 09:00
LOCAL: Sala de Reuniões do CONSEC
TÍTULO:

A TECHNOLOGICAL APPROACH TO INCREASE THE CYTOTOXIC EFFECT OF BULLFROG (Rana catesbeiana Shaw) OIL, in natura, AGAINST A2058 MELANOMA CELLS (AN in vitro EVIDENCE)

PALAVRAS-CHAVES:

Bullfrog oil, nanocapsules, nanoemulsion, microemulsion, anti-melanoma activity, cell necrosis.

PÁGINAS: 307
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
RESUMO:

The aim of this work was to investigate the in vitro cytotoxic effect of previously developed nanocapsules, nanoemulsion and microemulsion systems based on bullfrog oil (BFO) against human melanoma cells (A2058). The nanosystems were produced as described in previous studies and characterized according to droplet/particle distribution and zeta potential. The biocompatibility was evaluated by the determination of the hemolytic potential against human erythrocytes. The cytotoxicity assessment was made based on MTT and cell death assays, determination of the Reactive Oxygen Species (ROS) levels and cell uptake. The three nanosystems were successfully reproduced and showed hemolytic potential smaller than 10 % at all oil concentrations (50 and 100 µg.mL-1 ). The MTT assay revealed that the nanosystems decreased the mitochondrial activity up to 92 ± 2 %. The study showed that the free BFO induced cell apoptosis, while all nanostructured systems caused cell death by necrosis, associated to a ROS overproduction and increased ability to deliver the BFO across all cellular compartments (membrane, cytoplasm and nucleus). Finally, these results elucidate the in vitro BFO nanosystems cytotoxic effect against human melanoma cells, suggesting that these systems can be further investigated as safe and promising pharmaceutical products able to improve the therapeutic arsenal against melanoma.

MEMBROS DA BANCA:
Interna - 1199080 - ANA KATHERINE DA SILVEIRA GONCALVES DE OLIVEIRA
Externo à Instituição - ANSELMO GOMES DE OLIVEIRA - UNESP
Externo à Instituição - DANIEL CRISTIAN FERREIRA SOARES
Externa ao Programa - 1778262 - ELISSA ARANTES OSTROSKY
Presidente - 1178187 - ERYVALDO SOCRATES TABOSA DO EGITO