Banca de DEFESA: JEFFERSON DA SILVA BARBOSA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : JEFFERSON DA SILVA BARBOSA
DATA : 24/05/2019
HORA: 14:00
LOCAL: Departamento de Bioquímica
TÍTULO:

In vitro Immunostimulatory Activity of Sulfated Polysaccharides from Caulerpa cupressoides var. flabelata.


PALAVRAS-CHAVES:

Sulfated Polysaccharides; Immunostimulatory Activity; Macrophages; Inflammatory mediators.


PÁGINAS: 86
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
RESUMO:

Green seaweeds are rich sources of Sulfated Polysaccharides (SP) with potential biomedical and biotechnological applications. The aim of this work was to evaluate the immunostimulatory activity of PS from seaweed Caulerpa cupressoides var. flabelata on murine macrophages RAW264.7. PS were extracted and fractionated by acetone precipitation and evaluated for the ability to modify cell viability and to stimulate the production of inflammatory mediators such Nitric Oxide (NO), Intracellular Oxygen Reactive Species (ROS) and cytokines. Additionally, its effect on Nitric Oxide Synthase (iNOS) and Cyclooxygenase 2 (COX-2) genes expression, as well as the mode of interaction of PS and macrophages, was evaluated. The results revealed that the PS were not cytotoxic and were able to promote a strong increase in the production of NO, ERO and the cytokines Tumor Necrosis Factor alpha (TNF-α) and Interleukin 6 (IL-6). It was also observed that treatment with the PS increased the gene expression of  iNOS and COX-2. Through flow citometry and confocal microscopy was verified that SP was internalized and deposited near the nuclei of the cells. Together, these results indicate that C. cupressoides var flabelata PS have a strong immunostimulatory activity with potential biomedical applications


MEMBROS DA BANCA:
Presidente - 2195251 - HUGO ALEXANDRE DE OLIVEIRA ROCHA
Interna - 1490222 - SILVANA MARIA ZUCOLOTTO LANGASSNER
Externo ao Programa - 2275890 - MARCELO DE SOUSA DA SILVA
Externo à Instituição - DANIEL CHAVES DE LIMA - IFRN
Externa à Instituição - VALQUIRIA PEREIRA DE MEDEIROS - UFJF
Notícia cadastrada em: 14/05/2019 08:21
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