Banca de DEFESA: MAXSUELL LUCAS MENDES MARQUES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : MAXSUELL LUCAS MENDES MARQUES
DATA : 21/12/2018
HORA: 09:00
LOCAL: Sala Carl Peter von Dietrich
TÍTULO:

Sulfated polysaccharides from tropical seaweeds have anticoagulant activity: High anticoagulant sulfated galactans from green seaweed Udotea flabellum


PALAVRAS-CHAVES:

Tropical marine seaweeds; sulfated polysaccharides; antitumor; anti-IIa.


PÁGINAS: 37
GRANDE ÁREA: Ciências Biológicas
ÁREA: Bioquímica
RESUMO:

Many papers show the importance of anticoagulant compounds in the treatment of cancer, not only preventing blood clotting, but also inhibiting the activity of thrombin and factor Xa. These two molecules are activated by tumor factors and are involved in the proliferation and migration of tumor cells. Therefore, there is a search for anticoagulant agents that are inhibitors of these molecules. Seaweed synthesizes sulfated polysaccharides with several properties, including anticoagulant activity. Therefore, in this paper, sulfated polysaccharide-rich extracts were obtained from 21 tropical marine algae (4 red, 11 brown, and 6 green) found in northeastern Brazil and evaluated as anticoagulant agents. 15 extracts presented anticoagulant activity, including all extracts of green seaweed. Udotea flabellum extract was the most potent, which required only 3 µg to double the plasma coagulation time in the APTT test. Similar result was obtained with 1 µg of heparin. Two sulfated galactans with high anticoagulant activity were obtained from this extract using several bio-guided purification steps. These polysaccharides were named F-I (130 kDa) and F-II (75 kDa). They presented anticoagulant activity with intensity close to that of heparin. F-I and F-II (from 0.5 to 10 μg/mL) were not able to directly inhibit thrombin. In the presence of antithrombin, F-I (0.5 μg/mL) was more effective than heparin (0.5 μg/mL) as inhibitor of trombin, while F-II showed similar effect to heparin. Taken together, the results presented in the current study provide strong evidences of the anticoagulant/antitumor potential of the Udotea sulfated galactans and make these compound an interesting molecular tool to be used in future investigations that aimed at the design of new anticoagulant/antitumor agents.


MEMBROS DA BANCA:
Externo ao Programa - 2220417 - CARLOS AUGUSTO GALVAO BARBOZA
Presidente - 2195251 - HUGO ALEXANDRE DE OLIVEIRA ROCHA
Externo à Instituição - VALQUIRIA PEREIRA DE MEDEIROS - UFJF
Notícia cadastrada em: 11/12/2018 10:57
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