Banca de DEFESA: CHRISTIAN MELO DE OLIVEIRA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : CHRISTIAN MELO DE OLIVEIRA
DATA : 22/10/2019
HORA: 09:30
LOCAL: Sala de Reuniões do CONSEC
TÍTULO:

Hydrophobin-stabilized nanoemulsion as a promising delivery system for cancer cells.


PALAVRAS-CHAVES:

Hydrophobin, nanoemulsion, copaiba oil, stability, cancer.


PÁGINAS: 29
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
RESUMO:

Hydrophobin II (HFBII) is a protein able to produce emulsion systems that could be applied in the cancer therapy. This study reports the production of HFBII-stabilized oil-in-water nanoemulsion containing copaiba oil by the spontaneous emulsification method. HFBII was extracted by CO2, isolated by RP-HPLC, and characterized by electrophoresis, mass spectrometry and surface tension analyses. A 23 full factorial design was used to optimize the formulation, using HFBII, copaiba oil and organic-to-aqueous phase ratio as independent variables. The short-term stability of the nanoemulsion was investigated under different ionic strength and pH. In vitro cellular uptake of the nanoemulsion was evaluated in the human breast cancer (MCF-7) cells by flow cytometry. Isolated HFBII showed a molar mass of 7 kDa with a specific surface activity of 46.5 mN.m-1.µg-1. Optimal nanoemulsion formulation with droplet size of 154.2 ± 2.6, PDI of 0.153 ± 0.025 and zeta potential of -29.1 ± 3.2 was obtained using 0.005 wt% HFBII, 0.02 wt% copaiba oil and 10% (v/v) organic-to-aqueous phase ratio. Nanoemulsion was stable at low salt concentration (<25 mM) and pH close to neutrality. The HFBII-stabilized nanoemulsion was significantly internalized by MCF-7 cells. Moreover, HFBII is a promising biopolymer to stabilize nanoemulsion systems for the cancer therapy purposes.


MEMBROS DA BANCA:
Externo ao Programa - 1789788 - ADLEY ANTONINI NEVES DE LIMA
Externo à Instituição - ELQUIO ELEAMEN OLIVEIRA - UEPB
Presidente - 1178187 - ERYVALDO SOCRATES TABOSA DO EGITO
Notícia cadastrada em: 18/10/2018 15:00
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