Banca de QUALIFICAÇÃO: FERNANDO BASTOS PRESA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : FERNANDO BASTOS PRESA
DATA : 12/07/2018
HORA: 14:00
LOCAL: Sala Carl Peter von Dietrich
TÍTULO:

The protective role of sulfated polysaccharides from

green seaweed Udotea flabellum in cells exposed to

oxidative damage


PALAVRAS-CHAVES:

Sulfated galactan; 3T3 fibroblasts; green seaweed


PÁGINAS: 16
GRANDE ÁREA: Ciências da Saúde
ÁREA: Medicina
RESUMO:

Seaweed is a rich source of bioactive sulfated polysaccharides. We obtained six sulfated polysaccharide-rich fractions (UF-0.3, UF-0.5, UF-0.6, UF-0.7, UF-1.0, and UF-2.0) from the green seaweed Udotea flabellum (UF) by proteolytic digestion followed by sequential acetone precipitation. Biochemical analysis of these fractions showed that they were enriched with sulfated galactans. The viability and proliferative capacity of 3T3 fibroblasts exposed to FeSO4 (2 µM), CuSO4 (1 µM) or ascorbate (2 mM) was not affected. However, these cells were exposed to oxidative stress in the presence of FeSO4 or CuSO4 and ascorbate, which caused the activation of caspase-3 and caspase-9, resulting in apoptosis of the cells. We also observed increased lipid peroxidation, evaluated by the detection of malondialdehyde and decreased glutathione and superoxide dismutase levels. Treating the cells with the fractions protected the cells from the oxidative damage caused by the two salts and ascorbate. The most effective protection against the oxidative damage caused by iron was provided by UF-0.7 (1.0 mg/mL); on treatment with UF-0.7, cell viability was 55%. In the case of copper, cell viability on treatment with UF-0.7 was ~80%, but the most effective fraction in this model was UF-2.0, with cell viability of more than 90%. The fractions, mainly UF-0.7 and UF-2.0, showed low iron chelating activity, but high copper chelating activity and total antioxidant capacity (TAC). These results suggested that some of their protective mechanisms stem from these properties.


MEMBROS DA BANCA:
Externo à Instituição - DIEGO DE ARAUJO SABRY - IFRN
Presidente - 2195251 - HUGO ALEXANDRE DE OLIVEIRA ROCHA
Externo ao Programa - 2832746 - MONIQUE GABRIELA DAS CHAGAS FAUSTINO ALVES
Notícia cadastrada em: 21/06/2018 10:55
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