Banca de DEFESA: JOSE IVAN MARQUES DE MEDEIROS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : JOSE IVAN MARQUES DE MEDEIROS
DATA : 25/04/2018
HORA: 09:00
LOCAL: Sala 01 do do Programa de Pós-Graduação em Ciências Farmacêuticas
TÍTULO:

Tabernaemontana catharinensis: A phytochemical study and evaluation of anti-inflammatory activity in vivo.


PALAVRAS-CHAVES:

Inflammation; extract; patent


PÁGINAS: 90
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
RESUMO:

The use of plant extracts in the treatment of diseases is
widely diffused in Brazil, which can be explained by the
least at least because of the lower cost and the belief that such
products do not promote toxic effects. The species
Tabernaemontana catharinensis (Apocynaceae), the object of this
study, is popularly known as "goat's grain" and "weathervane",
and is native to South America. In Brazil, this species
has predominance in the south and northeast regions. Because it is a
popularly used to treat problems
inflammatory and lacking pharmacological evidence, the
The objective of this study was to evaluate the anti-inflammatory potential of
extracts and the fractions of the leaves of T. catharinensis in
in vivo and perform a phytochemical study of the extract. To this end, the
leaf extract was prepared by the maceration method
water: alcohol (70:30, v / v) and analyzed by Chromatography
Thin Layer and High Performance Liquid Chromatography
with high resolution mass detector with
electrospray. Part of the extract was submitted to the liquid-liquid partition
with increasing polarity solvent to obtain
fractions enriched in phenolic compounds. And after the analysis
phytochemistry was observed in the presence of glycosylated flavonoids
as major compounds in the extract and fractions. About the
pharmacological study, we used the edema models of
carrageenan-induced paw with pre and post treatment and bag
of zymosan-induced air, with post-treatment at doses (50,
100 and 150 mg / kg, orally). In the paw edema model,
evaluated the anti-nematode potential by decreasing the
edema and myeloperoxidase-MPO activity. For the model
of the air bag was evaluated the leukocyte migration (total and
differential), cytokine levels (IL-1 and IL-6) and MPO. As
As a result, in the paw edema model it was possible to observe
that the extract and fractions have anti-nematode potential in the
and post-treatment, presenting the best results for the
doses of 100 and 150 mg / kg (*** p <0.001, ** p <0.01,
respectively). The extracts and fractions presented
better results on the inhibition of MPO activity in the
after treatment. In the airbag model it was possible to observe
intense leukocyte inhibition (*** p <0.001) and inhibition of levels of
FEDERAL UNIVERSITY OF RIO GRANDE DO NORTE
HEALTH SCIENCES CENTER
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cytokines for both the extract and the (EtOAc fraction) in
dose 50 mg / kg. The extract and fractions did not present
cytotoxicity. The results demonstrate an anti-inflammatory potential
for extract and fractions of T. catharinensis, giving
support for the popular use of the species and providing subsidies for
development of an herbal medicine with anti-inflammatory properties.


MEMBROS DA BANCA:
Interno - 2195251 - HUGO ALEXANDRE DE OLIVEIRA ROCHA
Externo à Instituição - MARIANNA VIEIRA SOBRAL CASTELO BRANCO - UFPB
Presidente - 1490222 - SILVANA MARIA ZUCOLOTTO LANGASSNER
Notícia cadastrada em: 11/04/2018 15:21
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