Banca de QUALIFICAÇÃO: JULIANA FERNANDES DE CARVALHO
Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.DISCENTE : JULIANA FERNANDES DE CARVALHO
DATA : 01/08/2017
HORA: 14:00
LOCAL: sala de aula do PPGCSa
TÍTULO:
Oxacillin magnetically targeted for the treatment of Methicillin-Resistant S. aureus infection in rats
PALAVRAS-CHAVES:
Magnetic Field Therapy. Magnetite Nanoparticles. Methicillin-Resistant Staphylococcus aureus. Rats.
PÁGINAS: 10
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
RESUMO:
Purpose: To evaluate the effect of oxacillin bonded to magnetic nanoparticles in local infection model in rat.
Methods: Twelve Wistar rats weighing 290±18g were randomly divided into four groups (n=6, each) and all rats had a magnet ring sutured on their right thighs. In the biodistribution group rats 0.1mL of 99mTc-magnetite (0.66 MBq) was injected i.v and after 30 minutes, biodistribution of 99mTc-magnetite was evaluated in right and left thighs. The other groups were inoculated with MRSA in each thigh muscles. Group 1 rats were injected i.v. with magnetite, group 2 with Magnetite + Oxacillin, group 3 with saline twice a day. After 24 hours samples of muscle secretion were harvested for microbiological analysis; muscle, lungs and kidneys for histology.
Results: 99mTc-magnetite uptake was three-fold higher in right thigh muscles (with external magnet) than in the left. In magnetite and oxacillin-magnetite groups, bacterial/CFU was significantly lower in thigh muscles than in saline-controls. The inflammatory reaction in muscles and lungs was significantly lower in oxacillin-magnetite group-rats than in other groups (p<0.001).
Conclusion: This study confirms the potential antimicrobial activity of magnetic nanoparticles for Methicillin-Resistant S. aureus strains, which in addition to concentrate the antibiotic at the infection site, positively influenced the treatment.
MEMBROS DA BANCA:
Presidente - 6345702 - ARTUR DA SILVA CARRICO
Externo ao Programa - 1164475 - MAIZA ROCHA DE ABRANTES
Externo ao Programa - 2477216 - NAISANDRA BEZERRA DA SILVA FARIAS