Banca de QUALIFICAÇÃO: DAYANNE LOPES GOMES

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
DISCENTE : DAYANNE LOPES GOMES
DATA : 20/03/2017
HORA: 09:00
LOCAL: Sala Carl Von Peter Dietrich – Departamento de Bioquímica/CB/UFRN
TÍTULO:

Interaction of sulfated polysaccharides from green seaweed Caulerpa cupressoides var. flabellata with calcium oxalate crystals

PALAVRAS-CHAVES:

Urolithiasis; Green seaweed; Dumbbells COD; Plate-shaped COM.

PÁGINAS: 22
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
RESUMO:

Urolithiasis affects approximately 10% of the world population and is strongly associated with calcium oxalate (CaOx) crystals. There is currently no efficient compound that can be used to prevent this disease. However, seaweeds sulfated polysaccharides (SPs) have the ability to change the surface charge of the CaOx crystals and thus modify the crystallization dynamics, due to the interaction of the negative charges of this polymer with the surface of the crystal during its synthesis. We observed that the polysaccharide fractions of C. cupressoides modify the morphology and size of these crystals, as well as alter the surface charge of the crystals, making them negative, which may decrease the interaction of these crystals with the renal cells. Infrared analyzes have confirmed the binding of SPs and the thesis that they act not only by decreasing the Ca2+ supersaturation in the medium but also by interacting directly with the crystals. In addition, through an analysis of atomic composition (EDS), fluorescence miscroscopy and flow cytometry (SPs conjugated with FITC), an interaction model was proposed that schematizes the interactions between the Caulerpa SPs and the CaOx crystals to generate The morphologies of COD crystals rounded or in the form of dumbbells and with oval and flattened. This study is the first step in understanding the interactions between SPs, which are promising molecules for the treatment of urolithiasis, and the CaOx crystals that are the main cause of kidney stones.

MEMBROS DA BANCA:
Externo à Instituição - ARIANE FERREIRA LACERDA - UFRN
Presidente - 2195251 - HUGO ALEXANDRE DE OLIVEIRA ROCHA
Externo ao Programa - 1666310 - JOANA CRISTINA MEDEIROS TAVARES MARQUES