Banca de QUALIFICAÇÃO: CLARICE RIBEIRO CARDOSO

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : CLARICE RIBEIRO CARDOSO
DATE: 31/10/2022
TIME: 09:00
LOCAL: EAJ - Setor da graduação - Sala 04
TITLE:

MOLECULAR GENETIC CHARACTERIZATION OF PROVENANCE AND PROGENY OF Parkia platycephala Benth.

KEY WORDS:

faveira, ISSR marker, genetic diversity, ex situ conservation, seed orchards.

PAGES: 64
BIG AREA: Ciências Agrárias
AREA: Recursos Florestais e Engenharia Florestal
SUBÁREA: Silvicultura
SPECIALTY: Genética e Melhoramento Florestal
SUMMARY:

Parkia platycephala Benth. is a tree species native to transition areas between Cerrado and Caatinga in Brazil, used for various purposes, especially for animal feed due to its high forage potential. Its intense exploitation during the fruiting season, which coincides with the forage shortage period, represents a threat to its genetic conservation in its natural habitat, requiring the study of its genetic variability. Therefore, the objective of the research is to characterize the diversity and genetic structure existing in provenances and progenies of P. platycephala, by means of molecular marker ISSR, as a subsidy for the genetic conservation of the species and transformation of the test into a seed orchard. for seedlings. Sampling was carried out on 45 progenies established in a test of provenance and progenies at the Experimental Farm of the Federal University of Piauí, in Alvorada do Gurgueia, Piauí, Brazil. Genetic analyzes were performed on stem material and young leaves of the progenies of each provenance, with each progeny represented by 4 randomly selected individuals, totaling 180 plants. DNA from selected plants was extracted and amplifications were performed by PCR technique using 10 pre-selected ISSR primers. After obtaining the data, the efficiency of the tested primers was evaluated, based on the polymorphic information content (PIC), marker index (MI) and resolving power (PR), as well as the diversity and genetic structure of the provenances and progenies. Most of the polymorphic information contents tested were evaluated as being moderately informative (41≥PIC≤0.50), with mean values of 0.42, while the marker index (MI) and resolving power (RP) had values averages of 2.52 and 3.50, respectively. The diversity of Nei (H) presented an average of 0.315, and the Shannon index (I) average of 0.469. The analysis of the genetic structure showed a higher degree of specificity within the populations (81.87%), and the Φst value (0.13) confirms the low structure of procedures. results show a quality and efficiency of the feeders, which resulted in a detection of proven genetic diversity of P. platycephala.

COMMITTEE MEMBERS:
Presidente - 1715697 - FABIO DE ALMEIDA VIEIRA
Interno - 1721230 - MAURO VASCONCELOS PACHECO
Externa ao Programa - 1648735 - MARIA LUCIANA LIRA DE ANDRADE - UFRNExterna à Instituição - BRUNA ANAIR SOUTO DIAS - UFPI