Banca de QUALIFICAÇÃO: FRANCISCA JANEKELY BURITI

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : FRANCISCA JANEKELY BURITI
DATE: 18/03/2020
TIME: 09:30
LOCAL: Centro de Biociências, sala da pos-graduação do CB
TITLE:

DNA Barcode in Spondias from  Northeast: the specific case of S. bahiensis

KEY WORDS:

Spondias, DNA barcode, molecular phylogeny

PAGES: 58
BIG AREA: Ciências Agrárias
AREA: Recursos Florestais e Engenharia Florestal
SUBÁREA: Silvicultura
SPECIALTY: Genética e Melhoramento Florestal
SUMMARY:

Spondias bahiensis P. Carvalho, Van den Berg & M. Machado is one of the five species of Spondias genus present in Brazil, mainly occurring in the Northeast of Brazil. The plant was identified for many years as Spondias sp due to the doubts about the possibility of being a hybrid between Spondias tuberosa and Spondias mombin. S. bahiensis was identified based on molecular biology studies involving barcode markers that confirmed the plant as a new species. The objective of this study is to address the intraspecific variability of S. bahiensis species in relation to the morphological characteristics of the fruit and the fruit flavor and to resolve doubts related to the classification of the specie that could remain exist. We intend to investigate if the genetic variability in the species can be accessed with the available barcode markers. Methodology used consisted in collecting plant material (young leaves) from five species of Spondias, including S. bahiensis, at different locations in the states of Rio Grande do Norte and Paraíba, and carrying out PCR amplifications with universal barcodes markers rbcL, matK, trnG-trnS, trnH-psbA, rpoB, and rpoC. The intraspecific variabilaidae of S. bahiensis was studied from experimental plantings of EMPARN - Agricultural Research Company of Rio Grande do Norte in the municipality of Ipanguaçu, Rio Grande do Norte. The results obtained allowed the amplification and sequencing of the barcode regions by the different markers used and the realization of phylogenetic trees that confirm, in this analysis, the taxonomic position of S. bahiensis in comparison with the other species of the genus.

BANKING MEMBERS:
Presidente - 2257836 - PAULO SERGIO MARINHO LUCIO
Interna - 2294466 - CRISTIANE ELIZABETH COSTA DE MACEDO
Externo ao Programa - 3646062 - CARLOS ALFREDO GALINDO BLAHA