Banca de DEFESA: FABIANA SILVA DE ARAÚJO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : FABIANA SILVA DE ARAÚJO
DATE: 31/01/2020
TIME: 09:00
LOCAL: Centro de Biociências, sala da pos-graduação do CB
TITLE:

GENE EXPRESSION OF EUCALYPTUS THIOREDOXIN GENES IN RESPONSE TO SALT STRESS

KEY WORDS:

Keywords: Semi-quantitative RT-PCR, multigenic family, in silico analysis, functional analysis

PAGES: 60
BIG AREA: Ciências Agrárias
AREA: Recursos Florestais e Engenharia Florestal
SUBÁREA: Silvicultura
SPECIALTY: Genética e Melhoramento Florestal
SUMMARY:

Eucalyptus grandis is the first eucalyptus species that had its genome fully sequenced and published in 2014. The plant is of great economic interest for Brazil that currently has a planted area of 7.84 million hectares for pulp production. The species also remains the target of breeding studies through hybridization and genetic transformation for the production of some transgenic plants already released for experimental planting. The objective of this work is to progress in the functional characterization of genes coding for thioredoxin h in E. grandis. Specifically, we intend to study the gene expression product of 3 of these genes in a salt stress response condition in seedlings and in adult individuals maintained in experimental planting. The methodology used to obtain seedlings consists of subjecting pelletized seeds to plates containing moistened filter paper in three treatments, control, 50mM and 100mM NaCl. Adult plants are specimens of the TECHS (Tolerance of Clonal Eucalyptus to Hydric, Thermal and Biotic Stresses) experiments. RT-PCR assays are performed from extractions of total RNA from seedlings submitted to stresses and from adult plants of the TECHS. CDNA libraries are obtained from the total RNA extracted and PCR reactions with the oligonucleotide primers specific for the 3 genes studied are performed. Semi-quantitative gene expression is estimated on DNA electrophoresis gels. An in silico analysis of the promoter sequences of the genes studied is also performed to identify regulatory binding protein domains involved in the response to salt stress.
The results show that E. grandis seedlings respond to saline stress and are a good plant material for the functional analysis of plant genes. At the same time, there is a correlation between the stress response and the possible intervention of transcription factors linked to the type of stress employed here.

BANKING MEMBERS:
Interno - 2257836 - PAULO SERGIO MARINHO LUCIO
Externo ao Programa - 3646062 - CARLOS ALFREDO GALINDO BLAHA
Externo à Instituição - ALEK SANDRO DUTRA - UFC