Banca de QUALIFICAÇÃO: GLEYCE MEDEIROS DA SILVA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : GLEYCE MEDEIROS DA SILVA
DATE: 11/04/2025
TIME: 14:00
LOCAL: Google Meet
TITLE:

Phylogenomics and taxonomy of Symphypleona (Arthropoda: Hexapoda: Collembola): Is the reconstruction of evolutionary history through modern methodologies compatible with traditional systematics?   

KEY WORDS:

Eusymphypleona, edaphic fauna, phylogeny, genomic, springtails.   

PAGES: 112
BIG AREA: Ciências Biológicas
AREA: Biologia Geral
SUMMARY:

Collembola are terrestrial microarthropods considered good bioindicators of the soil health and have an important role in soil respiration processes. The class Collembola is composed of four orders (Entomobryomorpha, Poduromorpha, Symphypleona, and Neelipleona), which are currently accepted as independent orders. However, despite studies published in recent decades on the evolution and systematics of Collembola, the phylogenetic relationships among orders, and even among some families and subfamilies, remain largely uncertain, with few consensus. Symphypleona is one of the four orders of Collembola that has undergone various systematic changes over time. However, despite some speculations, its position within the class Collembola and the relationships among its internal groups remain uncertain. Due to this gap in knowledge about the order, this study aims to reconstruct the evolutionary history of Symphypleona based on molecular data and investigate its external and internal relationships. Additionally, it seeks to trace the morphological characters currently used to identify and distinguish taxa and describe potential new species.To achieve this, we categorized and identified material from the Collembola Collection of UFRN, as well as materials from partner researchers. Some of the material identified as new species was separated and prepared for taxonomic description following specific literature. Another part of the material was taken to Nanjing Agricultural University, where pre-sequencing procedures, such as DNA extraction, purification, and amplification, were performed. After sequencing, genomes were assembled, and Universal Single-Copy Orthologous Nuclear Genes (USCOs) and Ultra-Conserved Elements (UCEs) were extracted. Additionally, mitogenomes were assembled and annotated, and the 13 protein-coding genes will be used to produce the mitochondrial phylogeny.Bayesian and maximum likelihood phylogenetic inferences will be performed for USCOs, UCEs, and the 13 mitochondrial genes, and the consensus tree will be used as input for molecular clock estimation. Morphological character mapping will also be conducted, including the potential ancestral state.By carrying out this project, we expect to describe new taxa; obtain a phylogeny based on different molecular markers representative of Symphypleona to clarify the group's external and internal relationships; and determine which diagnostic characters have phylogenetic value 

 

COMMITTEE MEMBERS:
Interna - 3152261 - ANAMARIA DAL MOLIN
Presidente - 1879211 - BRUNO CAVALCANTE BELLINI
Externo à Instituição - RICARDO KOROIVA - UFPB