Banca de DEFESA: VALÉRIA COSTA DA SILVA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : VALÉRIA COSTA DA SILVA
DATE: 31/03/2025
TIME: 08:30
LOCAL: Remoto
TITLE:

EFEITO DO EXTRATO LIVRE E NANOPARTICULADO DAS FOLHAS DE Commiphora leptophloeos (MART.) J.B. GILLET. EM MODELOS DE INFLAMAÇÃO INTESTINAL E CÂNCER COLORRETAL QUIMICAMENTE INDUZIDOS

KEY WORDS:

Colitis; Colorectal cancer; Imburana; Burseraceae; Commiphora;

PAGES: 178
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Commiphora leptophloeos (Mart.) J.B. Gillet, from the Burseraceae family, is a plant
native to Brazil, found in the Caatinga, Cerrado, and Amazon biomes. It is popularly
known as imburana and is recognized for its medicinal properties in treating general
inflammation. Moreover, there are reports of its effects in alleviating gastrointestinal
symptoms such as colic and diarrhea. This plant contains important secondary
metabolites, including flavonoids such as orientin, vitexin, and quercetin, which have
recognized intestinal anti-inflammatory activity. Thus, this study aimed to evaluate the
effects of the free and nanoparticulated hydroethanolic extract (NPE) from C.
leptophloeos leaves in models of intestinal inflammation (preventive and curative) and
colitis-associated colorectal cancer (CAC). Additionally, this study included the
phytochemical evaluation of the extract and the development and characterization of a
nanoformulation containing the extract. Phytochemical annotation identified Cglycosylated flavonoids (quercetin, isovitexin, isoorientin, and orientin) and Oglycosylated flavonoids (quercetin-O-(O-galloyl)-hexose and quercetin-O-feruloyl-Ohexose), along with quinic acid. The total phenolic and flavonoid contents were 311.02 ±
0.03 mg/g and 174.90 ± 0.02 mg/g, respectively. The physicochemical characterization
of the NPE demonstrated a size of up to 100 nm, a PdI value of 0.13, stable Zeta potential,
and a significant reduction in the effective dose for regulating inflammation and
tumorigenesis signaling pathways—from 400 mg/kg with the free extract to only 4 mg/kg
with the NPE. Treatment with the free extract showed significant results (p<0.05) in
regulating inflammatory markers such as NF-κB p65/Cox-2, TNF-α, IL-1β, IL-6, Il-17,
and mTOR in a colitis model induced by 2,4-dinitrobenzenesulfonic acid (DNBS). The
free and nanoparticulated extract in a colitis model induced by dextran sulfate sodium
(DSS) regulated Tlr2/4, Jnk1/2, Cox-2, Tnf-α, Il-1β, Il-6, Il-17, Mip2, and Icam-1. In both
models, improvements in clinical symptoms and intestinal cytoarchitecture were
observed. Furthermore, both the free extract and NPE exhibited antitumor activity in the
CAC model, acting on important tumorigenesis signaling pathways such as Tp53, Ctnnb1,
Bax, and Bcl-2, reducing tumor volume, and increasing the abundance of the bacterium
Akkermansia muciniphila. In conclusion, C. leptophloeos exhibits both preventive and
curative intestinal anti-inflammatory activity in experimental models, as well as
antitumor activity. These findings support the potential development of a new
phytoingredient for the treatment of chronic inflammatory diseases.

 

COMMITTEE MEMBERS:
Externo à Instituição - EDNALDO GOMES DO NASCIMENTO - UFRN
Externa ao Programa - 2330188 - GERLANE COELHO BERNARDO GUERRA - UFRNExterna ao Programa - 1055045 - MARCELA ABBOTT GALVAO URURAHY - UFRNExterno à Instituição - RENAN OLIVEIRA SILVA DAMASCENO - UFPE
Presidente - 1490222 - SILVANA MARIA ZUCOLOTTO LANGASSNER
Externa à Instituição - TERESA VEZZA