Banca de DEFESA: JAMILE VITORIA ALVES FREIRE

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : JAMILE VITORIA ALVES FREIRE
DATE: 18/09/2025
TIME: 09:00
LOCAL: VIDEOCONFERÊNCIA
TITLE:

Development of Cosmetic Formulations Containing a Multicomponent

System with Ferulic Acid: Characterization and In Vitro Skin Release Tests

KEY WORDS:

Ferulic Acid. Multicomponent System. Antioxidant Activity. Cosmetic Formulation. SPF. In Vitro Skin Release.

PAGES: 135
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Phenolic compounds, such as ferulic acid (FA), are widely used in cosmetics due to their high

antioxidant activity and ability to protect against UV-induced damage, including skin

photoaging. However, their application is limited by low aqueous solubility and a tendency to

undergo rapid oxidation. To overcome these challenges, the multicomponent system

Cicloferulic® (CF) was developed, comprising FA, hydroxypropyl β-cyclodextrin

(HP-β-CD), and polyvinylpyrrolidone K30 (PVP K30). The system was characterized

physicochemically by DSC and TG/DTA, which demonstrated superior thermal stability of

CF compared to isolated FA. FTIR, SEM, and XRD analyses revealed molecular interactions

suggesting system formation and a transition from the crystalline to the amorphous state. In in

vitro cytotoxicity assays, FA exhibited toxicity at 5,000 µg/mL, whereas CF maintained cell

viability above 80% up to 10,000 µg/mL, showing toxicity only at 20,000 µg/mL. The FA

content incorporated into the system was quantified by UPLC, demonstrating high

incorporation efficiency and a yield of 92%. In antioxidant activity assays using a human

keratinocyte (HaCat) cell line, FA and CF showed similar activity at low concentrations, but

at higher concentrations, FA displayed pro-oxidant effects, whereas CF maintained high

antioxidant potential. In DPPH, ABTS, and CAT assays, CF exhibited greater antioxidant

activity than isolated FA; however, in cosmetic gel and emulsion formulations, efficacy was

reduced, possibly due to interactions between the complex and the aqueous vehicle. In in vitro

SPF assessments, formulations containing FA and CF initially showed higher values than

those with only sunscreen agents, but after 30 minutes of irradiation, a significant reduction in

SPF was observed, indicating no photostability effect. In in vitro cutaneous release studies,

formulations with isolated FA showed faster and higher release, while CF enabled slower and

controlled release, suggesting that complexation and formulation modulate FA availability

and flux. Cicloferulic® proved to be a promising strategy to overcome FA limitations,

offering enhanced stability, high antioxidant activity, and the potential for controlled and

sustained release of the active compound.

COMMITTEE MEMBERS:
Presidente - 1789788 - ADLEY ANTONINI NEVES DE LIMA
Externa ao Programa - 1679481 - ANA PAULA BARRETO GOMES - UFRNExterno à Instituição - ANDRÉ ROLIM BABY - USP
Externa ao Programa - 1778262 - ELISSA ARANTES OSTROSKY - null