Banca de DEFESA: IAGO DE SOUZA GOMES

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : IAGO DE SOUZA GOMES
DATE: 04/08/2025
TIME: 14:00
LOCAL: Google Meet
TITLE:

IN VIVO STUDY OF BIOMOLECULES ASSOCIATED WITH GLOMERULAR INJURY IN DIABETIC KIDNEY DISEASE

KEY WORDS:

Diabetic Kidney Disease; Urinary Extracellular Vesicles; Nephrin; Podocin; WT1.

PAGES: 92
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Diabetic Kidney Disease (DKD) is a common complication of Diabetes mellitus (DM), characterized by early podocyte loss and altered expression of key slit diaphragm proteins such as nephrin, podocin, and WT1. This study evaluated the mRNA expression of Nphs1, Nphs2, and Wt1 genes in the renal cortex, as well as the corresponding protein expression in renal tissue and urinary extracellular vesicles (uEVs), using an experimental model in Wistar rats. Twenty-three rats were distributed into three groups: control (C), diabetic induced by streptozotocin (D), and diabetic treated with insulin (DT), followed for 60 days. Biochemical parameters were assessed in serum and urine, in addition to gene expression by RT-qPCR and protein expression by Western blot.
After 60 days, insulin therapy effectively reduced blood glucose levels (p < 0.001) and albuminuria (albumin-to-creatinine ratio, ACR; p = 0.036) compared to the D group, although still elevated relative to controls (p = 0.034). Diabetic groups showed significantly increased mRNA expression of the studied genes (p = 0.001), while the corresponding protein levels were decreased in renal tissue (p = 0.021; 0.006; 0.004) and increased in uEVs (p = 0.034; 0.014; 0.004). Insulin therapy did not fully prevent or reverse these alterations, exhibiting a limited effect compared to the D group and remaining distinct from the control profile. These findings highlight the role of insulin to the control podocyte injury but also underscore its limitations when used alone in advanced stages of DKD. Overall, the results suggest that hyperglycemia induces a compensatory transcriptional response that fails to prevent protein loss and increased excretion via uEVs, contributing to DKD progression. Insulin treatment partially attenuates these changes, emphasizing the importance of glycemic control. Nephrin, podocin, and WT1, both in renal tissue and uEVs, emerge as promising sensitive and specific biomarkers for DKD monitoring.

 

COMMITTEE MEMBERS:
Presidente - 2323511 - ADRIANA AUGUSTO DE REZENDE
Interna - 1055045 - MARCELA ABBOTT GALVAO URURAHY
Interno - 1544647 - MATHEUS DE FREITAS FERNANDES PEDROSA
Externa ao Programa - 2477216 - NAISANDRA BEZERRA DA SILVA FARIAS - nullExterna à Instituição - RENATA CAROLINE COSTA DE FREITAS
Externo à Instituição - RENATO FERREIRA DE ALMEIDA JUNIOR - LGSD