Banca de DEFESA: JULIA DA COSTA SANTOS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : JULIA DA COSTA SANTOS
DATE: 31/03/2025
TIME: 14:00
LOCAL: VIDEOCONFERÊNCIA
TITLE:

PHYSICOCHEMICAL CHARACTERIZATION, CYTOTOXICITY AND

BIOACTIVE PROPERTIES OF SAPUCAIA NUT OIL (Lecythis pisonis)

KEY WORDS:

Lecythis pisonis, α-amylase, α-glucosidase, amyloglucosidase, antioxidant, Candida albicans.

PAGES: 97
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Lecythis pisonis, popularly known as sapucaia, is a plant species widely distributed in

Brazil. Its seeds, leaves and stem are traditionally used to treat diabetes, coughs, muscle pain

and itching, among other conditions. The aim of this study was to evaluate the physicochemical

characteristics, cytotoxicity and bioactive properties of sapucaia nut oil. The oil was

characterized using the techniques of hydrogen nuclear magnetic resonance and gas

chromatography coupled with mass spectrometry, revealing a high content of unsaturated fatty

acids, especially linoleic (53%) and oleic (25%) acids. The characterization also included

Fourier transform infrared spectroscopy (FTIR) and the evaluation of physical-chemical

parameters such as moisture, acidity, peroxide, saponification, iodine and density, all of which

confirmed the presence of unsaturated fatty acid chains and attested to the quality of the oil. In

addition, thermogravimetric analysis (TG/DTA) indicated high thermal stability, with

decomposition starting at 360.63°C. No cytotoxic activity was found in CHO-K1 (Chinese

hamster ovary cells) and HepG2 (human hepatocarcinoma cell line) cells. The oil had a total

phenolic concentration (TPC) of 291.77 µg EAG.g-1 and showed antioxidant activity against

ABTS radicals (2,2'-azinobis 3-ethylbenzothiazoline-6-sulfonic acid) of 4.28 µmol TE.g-1 and

DPPH (2,2-diphenyl-1-picrylhydrazyl) of 14.84 mMol TE.g-1 . The total antioxidant capacity

(CAT) of the methanolic fraction of the oil was 20.51 mg AA.g-1 . Sapucaia nut oil also showed

in vitro inhibitory capacity against the enzymes involved in carbohydrate digestion α-amylase

(94.01%), α-glucosidase (98.03%) and amyloglucosidase (84.88%). In addition, it showed

antifungal activity against Candida albicans with a minimum inhibitory concentration of 10

mg/mL. The results of this study contribute to understanding the physicochemical

characteristics, biological activities and potential applications of sapucaia oil, with the aim of

disseminating knowledge and encouraging interest in the food, pharmaceutical and cosmetics

industries. In addition, the aim is to enhance the value of the production chain, boost the local

economy and promote the preservation of the species, generating income opportunities for the

producing regions.

COMMITTEE MEMBERS:
Interna - 2378605 - CRISTIANE FERNANDES DE ASSIS
Presidente - 3652554 - FRANCISCO CANINDE DE SOUSA JUNIOR
Externa ao Programa - 1803301 - KATIA NICOLAU MATSUI - nullExterno à Instituição - SÉRGIO DANTAS DE OLIVEIRA JÚNIOR - INPA