CO-ENCAPSULATION OF Lactiplantibacillus plantarum AND BIOACTIVE
COMPOUNDS FROM JAMBOLAN (Syzygium cumini)
complex coacervation, Syzygium cumini (L.) Skeels, probiotics, Lactiplantibacillus plantarum, coencapsulated.
There is a growing demand for products containing probiotics and extracts rich in
phenolic compounds, whose combination allows simultaneous delivery of functional
and bioactive compounds in food, cosmetic and pharmaceutical products, providing
numerous benefits to the consumer. Jambolan, a fruit of the species Syzygium cumini,
has high levels of phenolic compounds, especially anthocyanins. In this context, the
objective of this study was to coencapsulate Lactiplantibacillus plantarum NRRL B-
4496 and the bioactive compounds extracted from the jambolan fruit by complex
coacervation. The jambolan extract obtained from the fruits showed values of 20.46 ±
1.38 mg EAG/g of total phenolic compounds, antioxidant activity by scavenging the
ABTS radical of 4354.32 ± 27.77 µmol TE/g and total antioxidant capacity of 604.54 ±
36.44 mg AA/g. The capsules were obtained by complex coacervation using gum
arabic, soy protein and soybean oil, and were subsequently lyophilized. Four systems
were developed, two with probiotic alone (ME-0%) and two associating probiotic and
jambolan extract at concentrations of 1% (ME-1%) and 5% (ME-5%). The
encapsulated systems containing jambolan extract showed higher probiotic
encapsulation efficiency (74.84 ± 3.13% for ME-1% and 71.16 ± 1.90% for ME-5%)
than the systems containing only the probiotic (57.45 ± 1.40% for ME-0%). In addition,
the anthocyanin encapsulation efficiency was 60.29 ± 3.08% for ME-1% and 60.84 ±
1.70% for ME-5%. The maximum yield and solubility of the encapsulated products
were 80.79 ± 1.48% and 11.11 ± 3.31%, respectively. The obtained microcapsules
presented particle size ranging from 12.23 to 19.85 µm and Zeta potential between -
2.59 ± 0.98 and - 18.35 ± 3.12 mV. Based on the results, the coencapsulation of L.
plantarum and jambolan fruit extract by complex coacervation, using encapsulating
materials of plant origin, demonstrated to be a viable strategy for obtaining a
multifunctional and vegan probiotic input.