Banca de DEFESA: VICENTE TOSCANO DE ARAUJO NETO

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : VICENTE TOSCANO DE ARAUJO NETO
DATE: 17/03/2023
TIME: 09:00
LOCAL: Link de acesso para videoconferência: https://meet.google.com/kxz-jucr-mdt?authuser=0
TITLE:
Molecular identification of Trypanosoma cruzi in domestic animals in
municipalities of the State of Rio Grande do Norte, Brazil

KEY WORDS:
Trypanosomatids, Trypanosoma cruzi, Leishmania spp, sheep, goats, horses, canids.

PAGES: 156
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:
The study evaluated the occurrence of natural infection by Trypanosoma cruzi and Leishmania
spp. using molecular markers and phylogenetic analysis in domesticated animals in the state of
Rio Grande do Norte. Blood clot samples were collected from 163 blood samples from sheep,
goats, horses, cattle and dogs and 64 aspirates from popliteal lymph nodes from dogs in nine
municipalities in the state of Rio Grande do Norte (RN). Initially, natural infection by T. cruzi
using 18S and kDNA was observed in samples of blood clots from sheep, goats and horses and
identified the parasite in 28.3% (17/60), 22.7% (5/22) and 15.4% (2/14) of the samples,
respectively. These animals were distributed in the three mesoregions studied throughout the
state of RN. At this time, infection in horses was reported for the first time in Brazil. Then, the
report of a dog in the acute phase of T. cruzi infection was related to clinical, hematological,
biochemical and parasitological aspects and characterization of the isolate by biological,
molecular and phylogenetic methods. An apparently asymptomatic young animal showed a
prolonged period of patent parasitemia, normocytic and hypochromic anemia. Associated with
this, the cardiac damage of this animal was observed by the high concentration of biomarkers
such as troponin I, NT-ProBN and qualitative segmental hypokinesia. Other samples of blood
clots from dogs were also subjected to T. cruzi kDNA amplification and showed 10.9% (7/64)
positivity. Phylogenetic analysis of ovine blood clot sample revealed DNA sequence with high
correlation with DTU II samples. While the parasite isolated from dogs in the acute phase was
genetically characterized by three markers and phylogenetic analysis as being TcIII. DTU III,
initially described in a wild environment, is increasingly present in the home cycle in the
Brazilian semi-arid region. Samples of popliteal aspirates were added in culture and the isolates
were also analyzed by molecular markers and phylogenetic analysis. Data with samples from
dogs revealed that cultures of the popliteus in biphasic medium were 12.5% (8/64) of positive
samples with flagellated forms similar to promastigotes. The sequencing and phylogenetic
analysis of eight culture samples showed a similarity above 99% with the Leishmania infantum
sequences. The wide distribution of infection by T.cruzi and Leishmania chagasi in
domesticated animals contributes to the expansion of the epidemiological scenario and the need
to better understand the role of these animals in the transmission cycle as reservoirs and/or
sentinels of infections in humans.

COMMITTEE MEMBERS:
Presidente - 1218940 - ANTONIA CLAUDIA JACOME DA CAMARA
Externo à Instituição - CARLOS ROBERTO ALVES - Fiocruz - RJ
Externa ao Programa - 1714243 - DANIELLA REGINA ARANTES MARTINS SALHA - UFRNExterno à Instituição - DAVID SOEIRO BARBOSA - UFMG
Externo à Instituição - VAGNER JOSE MENDONCA - UFPI
Notícia cadastrada em: 14/03/2023 15:40
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