Banca de DEFESA: MÁRCIA NATÁLIA DA SILVEIRA PINTO
Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : MÁRCIA NATÁLIA DA SILVEIRA PINTO
DATE: 24/02/2022
TIME: 09:00
LOCAL: Link de acesso para videoconferência: https://meet.google.com/exz-yejh-ntv
TITLE:
DEVELOPMENT OF A UHPLC-DAD METHOD FOR QUANTITATIVE AND SIMULTANEOUS DETERMINATION OF ACYLHYDRAZONE DERIVATIVES USING FACTORAL PLANNING
KEY WORDS:
Acylhydrazonic derivatives, versatile, UHPLC, FTIR, XRD, factorial plannin.
PAGES: 101
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:
Hydrazones are a group of very versatile and promising molecules, asthey have numerous
biological activities already reported in the literature. From this perspective, depending
on the type of replacement made in the structure, they may have anticancer, anti
inflammatory, antiviral, antimicrobial activity, among others. In this context, three
substances belonging to this class: ISVAN, ISBEN and ISANI were characterized by
different analytical techniques such as Fourier transform infrared spectroscopy (FTIR),
X-ray diffraction (XRD), thermogravimetry (TG) and ultraviolet liquid chromatography.
efficiency (UHPLC), in addition, an indicative method of stability was developed by
ultra-efficiency liquid chromatography with the aid of a 33 Box-Behenken factorial
design with the objective of developing an optimized method for quantification and
identification of substances in an efficient way. simultaneous. With the results obtained
from XRD and FTIR, it was possible to identify the crystalline nature of the molecules
and confirm the presence of the main functional groups present in the structure of the
substances, thus guaranteeing the molecular identity. Regarding the thermoanalytical
technique, it allowed to know the thermal behavior of the three acylhydrazonic derivatives
and for that, four heating rates were used (5,10,15 and 20 ºC min-1) under nitrogen
atmosphere, the profile of the DTG curve suggests that the mass loss for ISVAN and
ISBEN happens in a single step, while for ISANI the DTG curve suggests mass loss in
three steps, with the thermogravimetric curves it was also possible to carry out the
dynamic type kinetics study through the Ozawa method and with result was seen that
ISVAN has greater stability since it has higher activation energy. A forced degradation
study was also carried out under acidic and basic hydrolytic, oxidative, photostability,
and thermoanalytical conditions, as one of the parameters for validating the
chromatographic method developed, as well as for knowing the intrinsic stability of
substances and their degradation route.
BANKING MEMBERS:
Interno - 084.149.974-81 - ARTUR DE SANTANA OLIVEIRA - UFRN
Presidente - 1492900 - CICERO FLAVIO SOARES ARAGAO
Externa à Instituição - LILIAN GRACE DA SILVA SOLON - UNIFAP